Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

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Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

Name: Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E07947m

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Code: CSB-E07947m

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.9 ng/mL-60 ng/mL

Sensitivity: 0.22 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Name

serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

Alternative Names

Serpine1 ELISA Kit; Mr1 ELISA Kit; Pai1 ELISA Kit; Planh1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit

Abbreviation

SERPINE1

Uniprot NO.

P22777

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.9 ng/mL-60 ng/mL

Sensitivity

0.22 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	94
	Range %	88-102
1:8	Average %	96
	Range %	91-103

Recovery

The recovery of mouse PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	95	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	3.033	3.108	3.071	2.894
30	2.476	2.557	2.517	2.340
15	1.887	1.785	1.836	1.659
7.5	1.117	1.124	1.121	0.944
3.75	0.711	0.719	0.715	0.538
1.8	0.488	0.468	0.478	0.301
0.9	0.329	0.332	0.331	0.154
0	0.178	0.176	0.177

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Mouse SERPINE1 ELISA Kit was designed for the quantitative measurement of Mouse SERPINE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.22 ng/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. Involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.

Subcellular Location

Secreted.

Protein Families

Serpin family

Database Links

UNIGENE: Mm.250422

STRING: 10090.ENSMUSP00000039586

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