Mouse matrix metalloproteinase 1,MMP-1 ELISA kit

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Name: Mouse matrix metalloproteinase 1,MMP-1 ELISA kit
Brand: CUSABIO
SKU: CSB-E07417m

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Code: CSB-E07417m

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, tissue homogenates

Detect Range: 47 pg/mL-3000 pg/mL

Sensitivity: 11.75 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Target Background

Target Name

matrix metalloproteinase 1,MMP-1

Alternative Names

Mmp1a ELISA Kit; McolAInterstitial collagenase A ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1a ELISA Kit; MMP-1a ELISA Kit; Mcol-A ELISA Kit

Abbreviation

MMP-1

Uniprot NO.

Q9EPL5

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	91
	Range %	86-95
1:40	Average %	100
	Range %	97-103
1:80	Average %	91
	Range %	87-95
1:160	Average %	97
	Range %	93-100

Recovery

The recovery of mouse MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.482	2.510	2.496	2.317
1500	1.855	1.786	1.821	1.642
750	1.246	1.232	1.239	1.060
375	0.748	0.774	0.761	0.582
187.5	0.495	0.456	0.476	0.297
93.75	0.322	0.339	0.331	0.152
46.88	0.253	0.242	0.248	0.069
0	0.180	0.178	0.179

Troubleshooting and FAQs

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ELISA Data Analysis

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■ Customer Reviews

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Deletion of extracellular matrix metalloproteinase inducer/CD147 induces altered cardiac extracellular matrix remodeling in aging mice. Huet E. et al,/,2015
Changes in the expression and protein level of matrix metalloproteinases after exposure to waterpipe tobacco smoke Khabour OF. et al,/,2015
Protective Effect of Supercritical-Carbon Dioxide Fluid Extract from Flowers and Buds of Chrysanthemum indicum Linn&eacuten against UV-induced Photo-aging in Mice Zhang Xie et al,/,2015
Garlic Supplementation Ameliorates UV-Induced Photoaging in Hairless Mice by Regulating Antioxidative Activity and MMPs Expression Kim HK,/,2016
Photo-protective activity of pogostone against UV-induced skin premature aging in mice Wang XF.et al,/,2016
Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling Jiang L, et al,/,2019
Fermentation of Blackberry with L. plantarum JBMI F5 Enhance the Protection Effect on UVB-Mediated Photoaging in Human Foreskin Fibroblast and Hairless Mice through Regulation of MAPK/NF-κB Signaling Kim H R, et al,/,2019
Intentional endometrial injury enhances angiogenesis through increased production and activation of MMP-9 by TNF-α and MMP-3 in a mouse model YA Tu,/,2021
MitoQ combats tumor cell progression in Ehrlich ascites carcinoma mice: A crosstalk between mitochondrial oxidative status, mitophagy, and NF-κB signaling MA Oraby,/,2023
Neuropeptide Y, a potential marker for lupus, promotes lupus development DC Wang,/,2023
Single-Walled Carbon Nanotube-Guided Topical Skin Delivery of Tyrosinase to Prevent Photoinduced Damage J Ko,/,2023
In Vitro Biological Activities and Relative Chemicals in Different Extracts of Licorice and Fermenting Licorice Z Geng,/,/
Hybrid complex of tyrosinase and carbon-based materials for tissue engineering applications 고중현,/,2024
Protective effects of Colla Corii Asini Collagen Peptides on D-galactose injection combined with UVB irradiation-induced aging in mice Q Luo, S Zhang, Z Sun, Z Wang, Q Yue, X Sun, L Tian,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. Able to degrade synthetic peptides and type I and II fibrillar collagen.

Gene References into Functions

The difficulty to effectively maintain Mmp1a in the zymogen form may account for the tight control of Mmp1a expression and reduced expression in normal tissue as compared to inflammatory states or cancer. PMID:24737602
Stromal Mmp1a drives in vivo tumorigenesis. PMID:23708660
Matrix metalloproteinase Mmp-1a is not required for growth and fertility in mice and has a role in lung cancer progression by modulating inflammatory responses PMID:23548910

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

Peptidase M10A family

Database Links

UNIGENE: Mm.156952

KEGG: mmu:83995

STRING: 10090.ENSMUSP00000034492

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