Mouse factor B,BF ELISA Kit

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Code: CSB-E04695m
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma
Detect Range: 0.312 μg/mL-20 μg/mL
Sensitivity: 0.078 μg/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Alternative Names
Cfb ELISA kit; Bf ELISA kit; H2-BfComplement factor B ELISA kit; EC 3.4.21.47 ELISA kit; C3/C5 convertase) [Cleaved into: Complement factor B Ba fragment; Complement factor B Bb fragment] ELISA kit
Abbreviation
BF
Uniprot NO.
Species
Mus musculus (Mouse)
Sample Type
serum, plasma
Detection Range
0.312 μg/mL-20 μg/mL
Sensitivity
0.078 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Sandwich
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 96  
Range % 92-104  
1:200 Average % 95  
Range % 91-99  
1:400 Average % 97  
Range % 91-105  
1:800 Average % 102  
Range % 96-108  
Recovery
The recovery of mouse BF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 82-94  
EDTA plasma (n=4) 90 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
µg/ml OD1 OD2 Average Corrected  
20 2.712 2.811 2.762 2.608  
10 2.112 2.212 2.162 2.008  
5 1.428 1.447 1.438 1.284  
2.5 0.857 0.866 0.862 0.708  
1.25 0.524 0.512 0.518 0.364  
0.625 0.350 0.341 0.346 0.192  
0.312 0.268 0.263 0.266 0.112  
0 0.154 0.153 0.154    
Troubleshooting and FAQs
Images
  • Mouse factor B,BF ELISA Kit
Description

This Mouse factor B (BF) ELISA Kit was designed for the quantitative measurement of Mouse factor B (BF) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 μg/mL-20 μg/mL and the sensitivity is 0.078 μg/mL.

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function(From Uniprot)
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.
Subcellular Location
Secreted.
Protein Families
Peptidase S1 family
Database Links

UNIGENE: Mm.653

STRING: 10090.ENSMUSP00000025229

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