Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

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Code: CSB-E17817m

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.45 ng/mL-30 ng/mL

Sensitivity: 0.11 ng/mL

Trial Size:

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Target Background

Product Details

Alternative Names

F7 ELISA Kit; Cf7 ELISA Kit; Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit

Abbreviation

Uniprot NO.

P70375

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.45 ng/mL-30 ng/mL

Sensitivity

0.11 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Blood Coagulation

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅦ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	92
	Range %	86-96
1:2000	Average %	97
	Range %	92-102
1:4000	Average %	93
	Range %	88-97
1:8000	Average %	89
	Range %	84-94

Recovery

The recovery of mouse FⅦ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-95
EDTA plasma (n=4)	108	103-114

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.468	2.445	2.457	2.324
15	2.115	1.951	2.033	1.900
7.5	1.503	1.440	1.472	1.339
3.75	0.845	0.790	0.818	0.685
1.8	0.626	0.593	0.610	0.477
0.9	0.381	0.362	0.372	0.239
0.45	0.274	0.252	0.263	0.130
0	0.137	0.129	0.133

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Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

Description

This Mouse Coagulation factor VII (F7) ELISA Kit was designed for the quantitative measurement of Mouse Coagulation factor VII (F7) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.

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Target Background

Function(From Uniprot)

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.

Gene References into Functions

Leu8 is crucial for FVIIa-EPCR binding; this study characterizes its interaction in vivo in mice PMID:28035745
FVIIa-antithrombin but not FVIIa is a ligand for LRP1, and LRP1 contributes to the clearance of FVIIa-antithrombin in vivo PMID:27614059
FVIIa binding to EPCR leads to a barrier protective effect in vivo PMID:24977291
Murine FVIIa binds poorly to murine EPCR. PMID:22370814
GlycoPEGylated rFVIIa (N7-GP) has a prolonged hemostatic effect in hemophilic mice compared with rFVIIa PMID:21366858
Conclude that the fVII-targeted verteporfin photodynamic therapy that we report here is a novel and effective therapeutic with improved selectivity for the treatment of breast cancer. PMID:20504328
Recombinant FVIIa readily associates with the vascular endothelium and subsequently enters into extravascular spaces where it is likely to bind to tissue factor and is retained for extended time periods. PMID:19943873
true circadian rhythms for FVII were found PMID:15604416
Data suggest that long-term expression of murine activated factor VII is safe, but elevated levels cause premature mortality. PMID:18398505
FVIIa functions in the asthmatic response to an allergen by stimulating lung eosinophilia, airway hyperresponsiveness, and mucin production. PMID:19286924
Plasma elimination kinetics for factor VII are independent of its activation to factor VIIa and complex formation with plasma inhibitors. PMID:19404533