Mouse coagulation factor Ⅴ,FⅤ ELISA Kit

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Name: Mouse coagulation factor Ⅴ,FⅤ ELISA Kit
Brand: CUSABIO
SKU: CSB-E17815m

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Code: CSB-E17815m

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma

Detect Range: 78 ng/mL-5000 ng/mL

Sensitivity: 19.5 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

FVL, PCCF, activated protein c cofactor|coagulation factor V|coagulation factor V jinjiang A2 domain|factor V Leiden |coagulation factor 5,F5

Abbreviation

Uniprot NO.

O88783

Species

Mus musculus (Mouse)

Sample Type

serum, plasma

Detection Range

78 ng/mL-5000 ng/mL

Sensitivity

19.5 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Blood Coagulation

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅤ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-103
1:200	Average %	100
	Range %	95-104
1:400	Average %	95
	Range %	90-100
1:800	Average %	98
	Range %	94-105

Recovery

The recovery of mouse FⅤ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	88	84-93

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Description

This Mouse Coagulation factor V (F5) ELISA Kit was designed for the quantitative measurement of Mouse Coagulation factor V (F5) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

Gene References into Functions

Mice with the FVL mutation do not have increased spermatogenesis as compared to wildtype mice. PMID:28927238
Platelet-derived FV is a critical mediator of arterial thrombosis that involves platelet activation. PMID:28673898
Platelet-derived FV contributes to the control of angiogenesis and is likely associated with thrombin generation in hind limb ischemia model. PMID:27237401
Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of alpha1-antitrypsin. PMID:21795745
The FVL mutation does not influence coagulation activation, lung inflammation or survival in lethal influenza A. PMID:20413539
It suggested that there could be a combination of GLA deficiency and FVL or other thrombosis-related gene defect in patients with genetic severe early-onset thrombosis. PMID:20533258
that the murine platelet FV compartment is derived exclusively from primary biosynthesis within cells of marrow origin and an intact platelet FV pool is not required for protection from spontaneous hemorrhage or bleeding following minor trauma PMID:12816857
the murine platelet and plasma FV pools are biosynthetically distinct PMID:12855561
The source of the FVL leading to accelerated thrombosis appears to be circulating, non-platelet-derived plasma FVL. PMID:15809370
FVL has the ability to improve the hemophilia A or B phenotype, but this effect is principally evident at the microcirculation level following a particular vascular injury. PMID:16359510
observations demonstrate a synergistic interaction between alpha-galactosidase A deficiency and Factor V Leiden toward tissue fibrin deposition; concomitant mutations in these genes may increase the penetrance of vascular thrombotic events in humans PMID:16514103
Fetal gene defects precipitate platelet-mediated pregnancy failure in factor V Leiden mothers. PMID:17438064
The FVL mutation has no effect on the development of secondary tumours of colon cancer in livers of mice. PMID:17635646
FV Leiden allele has no effect in murine colitis and we thus question the importance of activated blood coagulation in the etiology or pathogenesis of inflammatory bowel disease. PMID:17849058
the factor V Leiden mutation is associated with enhanced thrombotic tendency after FeCl3 injury of the femoral artery on a standard fat diet, but not a high fat diet PMID:17938812
In contrast to humans, pregnancy induced a decrease in APC resistance in wild-type and in FV Leiden mice. PMID:19036061
Homozygous Factor V could promote atherosclerosis and fibrin deposition in apolipoprotein E deficient mice. PMID:19671356