Mouse choline acetyltransterase(ChAT)ELISA Kit

Promotion

Code: CSB-E17524m

Rate （N/A）
Review （0）
Citation （1）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates, cell culture supernates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Alternative Names

ChatCholine O-acetyltransferase ELISA kit; CHOACTase ELISA kit; ChAT ELISA kit; Choline acetylase ELISA kit; EC 2.3.1.6 ELISA kit

Abbreviation

CHAT

Uniprot NO.

Q03059

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates, cell culture supernates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ChAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	88-96
1:200	Average %	87
	Range %	82-91
1:400	Average %	103
	Range %	99-108
1:800	Average %	95
	Range %	90-100

Recovery

The recovery of mouse ChAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	92	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.042	2.136	2.089	1.933
500	1.407	1.481	1.444	1.288
250	0.893	0.947	0.920	0.764
125	0.610	0.658	0.634	0.478
62.5	0.475	0.463	0.469	0.313
31.2	0.321	0.305	0.313	0.157
15.6	0.254	0.249	0.252	0.096
0	0.159	0.152	0.156

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Images

Mouse choline acetyltransterase(ChAT)ELISA Kit

Description

This Mouse CHAT ELISA Kit was designed for the quantitative measurement of Mouse CHAT protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.