Mouse Thyroglobulin,TG ELISA Kit

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Code: CSB-E08241m

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96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 10 ng/mL-160 ng/mL

Sensitivity: 10 ng/mL

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Target Background

Product Details

Target Name

thyroglobulin

Alternative Names

Tg ELISA Kit; Tgn ELISA Kit; Thyroglobulin ELISA Kit; Tg ELISA Kit

Abbreviation

Uniprot NO.

O08710

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

10 ng/mL-160 ng/mL

Sensitivity

10 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-100
1:2	Average %	89
	Range %	82-100
1:4	Average %	93
	Range %	88-100
1:8	Average %	95
	Range %	89-105

Recovery

The recovery of mouse TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-110
EDTA plasma (n=4)	95	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
160	0.165	0.170	0.168
80	0.276	0.271	0.274
40	0.504	0.499	0.502
20	1.008	1.034	1.021
10	2.055	2.029	2.042

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Mouse Thyroglobulin,TG ELISA Kit

Description

Thyroglobulin (TG) is a large glycoprotein produced exclusively by thyroid follicular cells and serves as the precursor for thyroid hormone synthesis. This protein functions as a scaffold for the iodination and coupling reactions that generate triiodothyronine (T3) and thyroxine (T4) within thyroid follicles. Circulating thyroglobulin levels are commonly used as a biomarker in thyroid research and clinical diagnostics, particularly for monitoring thyroid function and detecting thyroid-related disorders. Changes in thyroglobulin expression and secretion are linked to various thyroid pathologies. This makes it a valuable target for studying thyroid physiology and disease mechanisms.

The Mouse Thyroglobulin ELISA Kit (CSB-E08241m) provides quantitative measurement of TG in Mus musculus samples. This competitive assay works with serum, plasma, and tissue homogenates. It has a detection range of 10 ng/mL to 160 ng/mL and a sensitivity of 10 ng/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, giving researchers a reliable tool for thyroglobulin quantification in mouse studies.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in thyroid-related research to quantify thyroglobulin levels in mouse models. The applications span endocrine studies examining thyroid function and autoimmune conditions affecting thyroid tissue.

• Autoimmune thyroid research: Measuring thyroglobulin antibody markers in serum samples from mouse models of thyroid autoimmunity
• Thyroid tissue analysis: Measuring intrathyroidal thyroglobulin concentrations alongside other thyroid hormones in tissue samples
• Endocrine profiling: Quantifying thyroglobulin as part of comprehensive thyroid function evaluation in experimental studies

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Citations

Thyroid dysfunction associated with follicular cell steatosis in obese male mice and humans Lee MH.et al,/,2015
Inflammatory stress promotes the development of obesity-related chronic kidney disease via CD36 in mice Yang P.et al,/,2017
2,3,5,4'-tetrahydroxy‑stilbene-2-O-β-D-glucoside attenuates methionine and choline‑deficient diet‑induced non‑alcoholic fatty liver disease Mingnuan Han.et al,/,2018
Puerarin Alleviates Experimental Autoimmune Thyroiditis by Regulating Macrophages Q Tao,/,2024

Target Background

Function(From Uniprot)

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.

Gene References into Functions

Findings suggest that coumestrol treatment may have some beneficial actions against thyroid-specific thyroglobulin (Tg) autoantibody production in the development of autoimmune thyroiditis through suppression of Th1 response. PMID:27384679
these data identify for the first time a Tg T cell epitope as a spontaneous target in ISAT. PMID:24879795
Final acquisition of secretory competence includes conformational maturation in the interval between linker and hinge segments of region I of thyroglobulin. PMID:21816825
The results showed that thyroglobulin message or protein is not expressed in placenta, decidua, or ovary in any stages of pregnancy. PMID:20236260
Findings demonstrate a novel aspect of murine and human thyroid autoimmunity, namely breaking B cell self-tolerance occurs first for Tg and subsequently for TPO. PMID:20573721
These results indicate that Runx2 deficiency in mice causes decreased Tg expression and a novel type of hypothyroidism. PMID:20375239
Development of iodine-accelerated autoimmune thyroiditis in NOD.H2h4 mice is not associated with enhanced iodine organification or differential B- or T-cell responses to iodinated determinants in Tg. PMID:20687399
specific properties of distinct Tg ChEL mutants may result in distinct patterns of Tg monomer folding PMID:20353937
Five novel autoimmune thyroiditis-causing T cell epitopes of thyroglobulin (Tgn) have been delineated and mapped with an algorithm that predicts Tgn immunopathogenic peptides containing I-A(k)-binding motifs. PMID:12391254
iodotyrosyl formation in normal thyroglobulin confers pathogenic potential to certain peptides that may otherwise remain innocuous and undetectable by conventional mapping methods PMID:16547286
Tg could initiate glomerular injury by reproducing the actions of TGF-beta1 in the mesangial cell. PMID:17495424
Data indicate that the R19K mutation induces local misfolding in the amino-terminal domain of Tg that has global effects on Tg transport and thyroid hormonogenesis. PMID:17916655
Together these results suggest that a unique low molecular weight variant of Tg is expressed in the kidney, where it could serve both physiological and pathological roles, including that of an autoantigen. PMID:19716808