Mouse Ornithine carbamoyltransferase, mitochondrial(OTC) ELISA kit

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Mouse Ornithine carbamoyltransferase, mitochondrial(OTC) ELISA kit

Name: Mouse Ornithine carbamoyltransferase, mitochondrial(OTC) ELISA kit
Brand: CUSABIO
SKU: CSB-EL017270MO

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Code: CSB-EL017270MO

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 3.12 mU/mL-200 mU/mL

Sensitivity: 0.78 mU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Name

ornithine carbamoyltransferase

Alternative Names

Otc ELISA Kit; Ornithine carbamoyltransferase ELISA Kit; mitochondrial ELISA Kit; EC 2.1.3.3 ELISA Kit; Ornithine transcarbamylase ELISA Kit; OTCase ELISA Kit

Abbreviation

OTC

Uniprot NO.

P11725

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

3.12 mU/mL-200 mU/mL

Sensitivity

0.78 mU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OTC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	91
	Range %	86-97
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-100

Recovery

The recovery of mouse OTC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-109
EDTA plasma (n=4)	96	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
200	2.348	2.272	2.310	2.196
100	1.826	1.732	1.779	1.665
50	1.176	1.239	1.208	1.094
25	0.708	0.743	0.726	0.612
12.5	0.409	0.431	0.420	0.306
6.25	0.257	0.271	0.264	0.150
3.12	0.199	0.204	0.202	0.088
0	0.115	0.112	0.114

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

This Mouse OTC ELISA Kit was designed for the quantitative measurement of Mouse OTC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 mU/mL-200 mU/mL and the sensitivity is 0.78 mU/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.

Gene References into Functions

Gene correction in adult OTC-deficient mice was lower and accompanied by larger deletions that ablated residual expression from the endogenous OTC gene, leading to diminished protein tolerance and lethal hyperammonemia on a chow diet PMID:26829317
The corresponding OTC tissue enzyme activities were between 3-6% of normal control in mouse and human liver. The use of the cryptic splice sites was reproduced in minigenes carrying murine or human mutant sequences PMID:25853564
Serum OCT seemed to reflect tumor necrosis factor-alpha-mediated hepatic damage in diabetic obese mice and could be useful in the application for non-alcoholic fatty liver disease with features of metabolic syndrome, such as obesity and diabetes. PMID:19793175
in vivo regulation by HNF4alpha PMID:11994307
Results highlight the importance of the interaction between the OTC spf-ash mutation (ornithine transcarbamylase deficiency) and genetic backgrounds on metabolic phenotypes (ureagenesis, arginine metabolism, and nitric oxide production). PMID:17925451
Phenotypic correction of OTC deficiency using low-dose helper-dependent adenoviral vectors is reported. PMID:18563850
Lysine 88 acetylation negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals. PMID:19318352