Mouse Apolipoprotein C-II(APOC2) ELISA kit

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Name: Mouse Apolipoprotein C-II(APOC2) ELISA kit
Brand: CUSABIO
SKU: CSB-EL001932MO

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Code: CSB-EL001932MO

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 18.8 ng/mL-1200 ng/mL

Sensitivity: 9.4 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Target Name

apolipoprotein C-II

Alternative Names

Apoc2Apolipoprotein C-II ELISA kit; Apo-CII ELISA kit; ApoC-II ELISA kit; Apolipoprotein C2 ELISA kit

Abbreviation

APOC2

Uniprot NO.

Q05020

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

18.8 ng/mL-1200 ng/mL

Sensitivity

9.4 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	97
	Range %	88-102
1:40	Average %	93
	Range %	85-98
1:80	Average %	103
	Range %	96-107
1:160	Average %	104
	Range %	95-109

Recovery

The recovery of mouse APOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	101	94-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1200	0.094	0.097	0.096
600	0.229	0.221	0.225
300	0.429	0.444	0.437
150	0.707	0.726	0.717
75	1.109	1.068	1.089
37.5	1.535	1.474	1.505
18.8	1.682	1.639	1.661
0	2.048	1.967	2.008

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Mouse APOC2 ELISA Kit was designed for the quantitative measurement of Mouse APOC2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 18.8 ng/mL-1200 ng/mL and the sensitivity is 9.4 ng/mL.

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Mice lacking prostaglandin E receptor subtype 4 manifest disrupted lipid metabolism attributable to impaired triglyceride clearance Cai Y. et al,/,2015
Methionine adenosyltransferase 1a antisense oligonucleotides activate the liver-brown adipose tissue axis preventing obesity and associated hepatosteatosis D Sáenz de Urturi,/,2022
Methionine adenosyltransferase 1a antisense oligonucleotides activate the liver-brown adipose tissue axis preventing obesity and associated hepatosteatosis D Saenz de Urturi Indart,/,2022

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase.

Subcellular Location

Secreted.

Protein Families

Apolipoprotein C2 family

Tissue Specificity

Adult and fetal liver, intestine and peritoneal macrophages.

Database Links

UNIGENE: Mm.28394

KEGG: mmu:105886299

STRING: 10090.ENSMUSP00000003074

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