Human protein disulfide isomerase,PDI ELISA Kit

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Name: Human protein disulfide isomerase,PDI ELISA Kit
Brand: CUSABIO
SKU: CSB-E09003h

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Code: CSB-E09003h

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.312 ng/mL-20 ng/mL

Sensitivity: 0.078 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

Cellular thyroid hormone binding protein ELISA Kit; Cellular thyroid hormone-binding protein ELISA Kit; Collagen prolyl 4 hydroxylase beta ELISA Kit; Disulphide Isomerase ELISA Kit; DSI ELISA Kit; EC 5.3.4.1 ELISA Kit; Endoplasmic reticulum resident protein 59 ELISA Kit; ER protein 59 ELISA Kit; ERBA2L ELISA Kit; ERp59 ELISA Kit; GIT ELISA Kit; Gltathione insulin transhydrogenase ELISA Kit; Glutathione insulin transhydrogenase ELISA Kit; P4HB ELISA Kit; P4Hbeta ELISA Kit; p55 ELISA Kit; PDI ELISA Kit; PDIA1 ELISA Kit; PDIA1_HUMAN ELISA Kit; PDIR ELISA Kit; PHDB ELISA Kit; PO4DB ELISA Kit; PO4HB ELISA Kit; Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) ELISA Kit; Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit ELISA Kit; PROHB ELISA Kit; Prolyl 4 hydroxylase beta polypeptide ELISA Kit; Prolyl 4 hydroxylase beta subunit ELISA Kit; Prolyl 4 hydroxylase subunit beta ELISA Kit; Prolyl 4-hydroxylase subunit beta ELISA Kit; Protein disulfide isomerase associated 1 ELISA Kit; Protein disulfide isomerase, family A, member 1 ELISA Kit; Protein disulfide isomerase/oxidoreductase ELISA Kit; Protein disulfide-isomerase ELISA Kit; Protocollagen hydroxylase ELISA Kit; Thbp ELISA Kit; Thyroid hormone binding protein p55 ELISA Kit; Thyroid hormone binding protein p55 cellular ELISA Kit; V erb a avian erythroblastic leukemia viral oncogene homolog 2 like ELISA Kit

Abbreviation

P4HB

Uniprot NO.

P07237

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PDI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	95
	Range %	89-99
1:10	Average %	98
	Range %	93-103
1:20	Average %	92
	Range %	88-96
1:40	Average %	95
	Range %	87-99

Recovery

The recovery of human PDI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-101
EDTA plasma (n=4)	88	82-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.724	2.587	2.656	2.518
10	2.125	2.008	2.067	1.929
5	1.368	1.397	1.383	1.245
2.5	0.842	0.831	0.837	0.699
1.25	0.526	0.538	0.532	0.394
0.625	0.345	0.334	0.340	0.202
0.312	0.262	0.254	0.258	0.120
0	0.139	0.137	0.138

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Human Protein Disulfide Isomerase (PDI) ELISA Kit is an essential tool for quantitative detection of PDI in human serum, plasma, and tissue homogenates. This ELISA kit is specifically designed for research in the field of cancer and cardiovascular, allowing researchers to accurately measure PDI levels with a high level of sensitivity and a wide detection range.

The PDI protein, also known as protein disulfide-isomerase, plays a crucial role in protein folding and disulfide bond formation, making it a key player in various cellular processes. Our PDI ELISA kit is based on a sandwich assay principle, providing reliable and reproducible results with minimal interference from other proteins.

With a sensitivity of 0.078 ng/mL and a detection range of 0.312 ng/mL-20 ng/mL, this ELISA kit can accurately measure PDI levels in a variety of samples. The assay time ranges from 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, ensuring accurate and precise measurement of PDI levels.

This ELISA kit is an excellent choice for researchers studying cancer and cardiovascular. By providing accurate and sensitive measurements of PDI levels, our ELISA kit can help researchers gain a deeper understanding of the role of PDI in cancer and potentially identify new therapeutic targets.

■ Customer Reviews

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P4HB maintains Wnt-dependent stemness in glioblastoma stem cells as a precision therapeutic target and serum marker Z Yuan, H Jing, Y Deng, M Liu, T Jiang, X Jin, W Lin,/,2024

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

Gene References into Functions

Peroxynitrite preferentially oxidizes the dithiol redox motifs of protein-disulfide isomerase. PMID:29191937
Overexpression of HIF-1alpha and P4HB is associated with poor prognosis in patients with gastric cancer. PMID:29904245
Study demonstrated that the expression of P4HB is frequently upregulated at the mRNA and protein levels in diffuse gliomas. Its high expression was significantly correlated with high Ki-67, more TP53 mutations and poor survival outcome. These findings imply that high expression of P4HB plays an important role in diffuse glioma progression. PMID:29207176
identify a potent and selective PDIA1 inhibitor, KSC-34, with 30-fold selectivity for the a site over the a' site. KSC-34 displays time-dependent inhibition of PDIA1 reductase activity in vitro with a kinact/ KI of 9.66 x 10(3) M(-1) s(-1) and is selective for PDIA1 over other members of the PDI family, and other cellular cysteine-containing proteins. PMID:29521097
P4HB promotes hepatocellular carcinoma progression by down-regulating GRP78 expression and subsequently promoting epithelial-to-mesenchymal transition. PMID:28052026
analysis of antiplatelet activity of CxxC through binding to Cys400 in the PDI a0 domain, which can be further exploited as a model for sitedriven antithrombotic agent development PMID:28109047
Current findings indicate that thiol isomerase-mediated disulfide bond modification in receptors and plasma proteins is an important layer of control of thrombosis and vascular function more generally. PMID:28598864
DIA1 was robustly secreted by physiological levels of arterial laminar shear in endothelial cells and supported alpha 5 integrin thiol oxidation. PMID:28034831
Kinetic-based trapping by intervening sequence variants of the active sites of protein-disulfide isomerase identifies platelet protein substrates. PMID:28364042
a mechanism of dual Ero1alpha regulation by dynamic redox interactions between PDI and the two Ero1alpha flexible loops that harbor the regulatory cysteines. PMID:27703014
analysis of how redox affects human protein disulfide isomerase regulate binding affinity of 17 beta-estradiol PMID:28257787
These findings improve our understanding of PDI-protected aggregation of wild-type alpha-Syn and its H50Q familial mutant. PMID:27142583
Association of P4HB polymorphisms with sporadic amyotrophic lateral sclerosis susceptibility in the Chinese Han population. PMID:26000911
the effect of the endoplasmic reticulum chaperone protein disulfide isomerase (PDI) on beta-cell dysfunction, was examined. PMID:26607804
Amyotrophic lateral sclerosis-linked PDIA1 mutations disrupt motor neuron connectivity. PMID:26869642
direct binding of PDIA1 to VWF, is reported. PMID:26670633
Selective sequestration of PDI1A in a calcium depletion-mediated complex with the abundant chaperone calreticulin attenuates the effective concentration of this major lumenal thiol oxidant. PMID:25575667
Cole-Carpenter syndrome is caused by a specific de novo mutation in P4HB that impairs the disulfide isomerase activity of protein disulfide isomerase. PMID:25683117
PDI has a role as a competent regulator and a specific substrate of Ero1alpha govern efficient and faithful oxidative protein folding and maintain the ER redox homeostasis PMID:25258311
The crystal structure of the dimeric form of noncatalytic bb' domains of human PDIA1 determined to 2.3 A resolution revealed that the formation of dimers occludes the substrate binding site. PMID:24549644
Elevated P4HB expression is associated with temozolomide resistance in malignant glioma. PMID:23444257
). The results suggest that P4HB is a modifier gene inamyotrophic lateral sclerosis susceptibility and may represent a potential therapeutic target for amyotrophic lateral sclerosis . PMID:23337974
A mixed disulfide complex was formed with the catalytic domain A1 from human PDI consistent with a model for cotranslational oxidative protein folding wherein PDI acts as a placeholder that is relieved by the pairing of cysteines caused by substrate folding. PMID:23141538
TPM4, PDIA and SRC8 were also localized to the trophoblast cells, further highlighting the importance of these cytoskeletal remodelling proteins in early pregnancy PMID:21373848
these data revealed a redox-regulated chaperone function of PDI in delivering antigenic peptides from TAP to MHC-I. PMID:21299467
Functional PDI is rapidly secreted from human umbilical vein endothelial cells in culture upon activation with thrombin or after laser-induced stimulation. PMID:20668226
domain c is required for the stabilization and maintenance of the chaperone function of PDI under extreme conditions PMID:15358778
data indicate that binding sites in three PDI domains, a, b', and a', contribute to efficient C-P4H tetramer assembly PMID:15590633
PDI can be S-nitrosated and PDI-SNO can be denitrosated by PDI suggesting that this enzyme could be intimately involved in the transport of intracellular NO equivalents to the cell surface. PMID:15611098
Data show that protein disulfide isomerase can switch its conformation from dimer to tetramer in its functions as a foldase. PMID:15695804