Human p53/tumor protein,p53/TP53 ELISA Kit

Product Details

Customer Reviews and Q&A

Target Background

Name: Human p53/tumor protein,p53/TP53 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08334h-IS

Promotion

Code: CSB-E08334h-IS

Rate （N/A）
Review （0）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, cell culture supernates, cell lysates, tissue homogenates

Detect Range: 62.5 pg/mL-4000 pg/mL

Sensitivity: 31.25 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Alternative Names

Antigen NY-CO-13 ELISA Kit; BCC7 ELISA Kit; Cellular tumor antigen p53 ELISA Kit; FLJ92943 ELISA Kit; LFS1 ELISA Kit; Mutant tumor protein 53 ELISA Kit; p53 ELISA Kit; p53 tumor suppressor ELISA Kit; P53_HUMAN ELISA Kit; Phosphoprotein p53 ELISA Kit; Tp53 ELISA Kit; Transformation related protein 53 ELISA Kit; TRP53 ELISA Kit; tumor antigen p55 ELISA Kit; Tumor protein 53 ELISA Kit; Tumor protein p53 ELISA Kit; Tumor suppressor p53 ELISA Kit

Abbreviation

TP53

Uniprot NO.

P04637

Species

Homo sapiens (Human)

Sample Type

serum, plasma, cell culture supernates, cell lysates, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human p53/TP53 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	101
	Range %	94-108
1:4	Average %	95
	Range %	88-102
1:8	Average %	91
	Range %	84-97

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

4000

2.746

2.634

2.690

2.545

2000

1.653

1.667

1.660

1.515

1000

1.052

1.028

1.040

0.895

500

0.664

0.629

0.646

0.501

250

0.384

0.391

0.387

0.242

125

0.271

0.253

0.262

0.116

62.5

0.205

0.197

0.201

0.056

0.146

0.145

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This ELISA kit provides quantitative measurement of human TP53 (p53) protein in research samples, supporting studies in cancer biology where p53 status directly influences tumor suppression, apoptotic signaling, and therapeutic response. Because p53 is predominantly an intracellular protein that accumulates upon DNA damage or stabilizing mutations, detecting it across both secreted and lysate-based matrices requires a kit designed for diverse sample inputs.

Detection Range: The 62.5–4000 pg/mL range captures the elevated p53 levels characteristic of cells harboring missense mutations that stabilize the protein, while accommodating the lower concentrations found in wild-type expressing tissues. Samples from tumors with high mutant p53 accumulation may require dilution to fall within range.

Sensitivity: At 31.25 pg/mL, the kit detects p53 in conditions where protein levels are low, such as wild-type p53-expressing cells under basal conditions or dilute cell culture supernatants containing released p53 following apoptotic events.

Sample Compatibility: Validation across serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates enables researchers to correlate circulating p53 levels with intracellular accumulation in matched tumor and blood samples from the same subjects.

Assay Efficiency: The 1–5 hour protocol using only 50–100 µL accommodates precious clinical biopsies and primary tumor-derived cultures where material is limited.

This kit is directly applicable to studies of p53-driven tumorigenesis, chemoresistance profiling, and Li-Fraumeni syndrome research.

■ Customer Reviews

Submit a Review here

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.

Gene References into Functions

Study summarizes the different functions of p53 in adipocyte development and in adipose tissue homeostasis. Furthermore, It explores the manipulation of p53 levels in adipose tissue depots and the impact on systemic energy metabolism in the context of insulin resistance and obesity. [review] PMID:30181511
a USP15-dependent lysosomal pathway controls p53-R175H turnover in ovarian cancer cells PMID:29593334
Results indicate that the underlying mechanisms whereby etoposide and ellipticine regulate CYP1A1 expression must be different and may not be linked to p53 activation alone. PMID:29471073
Studied association of tumor protein p53 and drug metabolizing enzyme polymorphisms with clinical outcome in patients with advanced nonsmall cell lung cancer. PMID:28425245
POH1 knockdown induced cell apoptosis through increased expression of p53 and Bim. PMID:29573636
a heretofore unappreciated effect of chronic high fat diet on beta-cells, wherein continued DNA damage owing to persistent oxidative stress results in p53 activation and a resultant inhibition of mRNA translation. PMID:28630491
diffuse large B cell lymphoma lacking CD19 or PAX5 expression were more likely to have mutant TP53. PMID:28484276
that proliferation potential-related protein promotes esophageal cancer cell proliferation and migration, and suppresses apoptosis by mediating the expression of p53 and IL-17 PMID:30223275
Infection of HIV-1 and subsequent HIV-1 reverse transcription are inhibited in HCT116 p53(+/+) cells in comparison to HCT116 p53(-/-) cells. Tumor suppressor gene p53 expression is upregulated in non-cycling cells. The restrictions of HIV by p53 is associated with the suppression of ribonucleotide reductase R2 subunit expression and phosphorylation of SAMHD1 protein. PMID:29587790
It has been shown that MDM2 and MDMX are targetable vulnerabilities within TP53-wild-type T-cell lymphomas. PMID:29789628
A significant increase in the expression of p53 and Bax was observed in cells treated with alpha-spinasterol, while cdk4/6 were significantly down-regulated upon exposure to alpha-spinasterol. PMID:29143969
There was a significant correlation between telomere dysfunction indices, p53, oxidative stress indices, and malignant stages of GI cancer patients PMID:29730783
PGEA-AN modulates P53 system which further leads to the death of the neuroblastoma cells with no effect on renal system in vivo owing it to be a future prospect for development of anticancer moiety against neuroblastoma. PMID:29644528
these data indicate that activation of autophagy reduces expression of STMN1 and p53, and the migration and invasion of cancer cells contributes to the anti-cancer effects of the Halofuginone. These findings may provide new insight into breast cancer prevention and therapy. PMID:29231257
miR-150 suppresses cigarette smoke-induced lung inflammation and airway epithelial cell apoptosis, which is causally linked to repression of p53 expression and NF-kappaB activity. PMID:29205062
tumors harboring TP53 mutations, which can impair epithelial function, have a unique bacterial consortium that is higher in relative abundance in smoking-associated tumors. PMID:30143034
crosstalk among p53, lipid metabolism, insulin resistance, inflammation and oxidative stress has roles in Non-alcoholic fatty liver disease [review] PMID:30473026
Ubiquitin-conjugating enzyme E2S (UBE2S) enhanced the ubiquitination of p53 protein to facilitate its degradation in hepatocellular carcinoma (HCC) cells. PMID:29928880
p53 knockout compensates osteopenia in murine Mysm1 deficiency. PMID:29203593
SIRT1 had a pivotally protective role in the regulation of ADSCs aging and apoptosis induced by H2O2 PMID:29803744
133p53 promotes tumour invasion via IL-6 by activation of the JAK-STAT and RhoA-ROCK pathways. PMID:29343721
mutant TP53 G245C and R273H can lead to more aggressive phenotypes and enhance cancer cell malignancy. PMID:30126368
PD-L1, Ki-67, and p53 staining individually had significant prognostic value for patients with stage II and III colorectal cancer PMID:28782638
This study of patients with ccRCC, pooled analysis and multivariable modeling demonstrated that three recurrently mutated genes, BAP1, SETD2, and TP53, have statistically significant associations with poor clinical outcomes. Important clinical confounders, mutations of TP53 and SETD2 were associated with decreased CSS and RFS, respectively. PMID:28753773
Study revealed that the Wnt/beta-catenin signaling pathway and its major downstream target, c-Myc increased the miR552 levels and miR552 directly targets p53 tumor suppressor. miR552 may serve as an important link between functional loss of APC, leading to abnormal Wnt signals, and the absence of p53 protein in colorectal cancer. PMID:30066856
High levels of glucose leads to endothelial dysfunction via TAF1-mediated p53 Thr55 phosphorylation and subsequent GPX1 inactivation. PMID:28673515
Although tumor protein p53 (p53) does not directly control the luminal fate, its loss facilitates acquisition of mammary stem cell (MaSC)-like properties by luminal cells and predisposes them to development of mammary tumors with loss of luminal identity. PMID:28194015
Fifty-two percent of patients diagnosed with glioma/glioblastoma with a positive TP53 mutation. PMID:29454261
the expression of Ser216pCdc25C was also increased in the combined group, indicating that irinotecan likely radiosensitized the p53-mutant HT29 and SW620 cells through the ATM/Chk/Cdc25C/Cdc2 pathway. PMID:30085332
In the former, p53 binds to the CDH1 (encoding E-cadherin) locus to antagonize EZH2-mediated H3K27 trimethylation (H3K27me3) to maintain high levels of acetylation of H3K27 (H3K27ac). PMID:29371630
Among the hits, miR-596 was identified as a regulator of p53. The overexpression of miR-596 significantly increased p53 at the protein level, thereby inducing apoptosis. PMID:28732184
Apoptosis pathways are impaired in fibroblasts from patients with SSc, leading to chronic fibrosis. Nonetheless, PUMA/p53 pathway may not be involved in dysfunction of apoptosis mechanisms in fibroblasts of patients with SSc. PMID:28905491
Low TP53 expression is associated with drug resistance in colorectal cancer. PMID:30106452
The activation of p38 in response to low doses of ultraviolet radiation was postulated to be protective for p53-inactive cells. Therefore, MCPIP1 may favor the survival of p53-defective HaCaT cells by sustaining the activation of p38. PMID:29103983
TP53 missense mutations are associated with castration-resistant prostate cancer. PMID:29302046
P53 degradation is mediated by COP1 in the breast cancer. PMID:29516369
Combined inactivation of the XRCC4 non-homologous end-joining (NHEJ) DNA repair gene and p53 efficiently induces brain tumours with hallmark characteristics of human glioblastoma. PMID:28094268
A direct link between Y14 and p53 expression and suggests a function for Y14 in DNA damage signaling. PMID:28361991
TP53 Mutation is associated with Mouth Neoplasms. PMID:30049200
Cryo-Electron Microscopy studies on p53-bound RNA Polymerase II (Pol II) reveal that p53 structurally regulates Pol II to affect its DNA binding and elongation, providing new insights into p53-mediated transcriptional regulation. PMID:28795863
Increased nuclear p53 phosphorylation and PGC-1alpha protein content immediately following SIE but not CE suggests these may represent important early molecular events in the exercise-induced response to exercise PMID:28281651
E6/E7-p53-POU2F1-CTHRC1 axis promotes cervical cancer cell invasion and metastasis PMID:28303973
accumulated mutant-p53 protein suppresses the expression of SLC7A11, a component of the cystine/glutamate antiporter, system xC(-), through binding to the master antioxidant transcription factor NRF2. PMID:28348409
Consistently, forced expression of p53 significantly stimulated ACER2 transcription. Notably, p53-mediated autophagy and apoptosis were markedly enhanced by ACER2. Depletion of the essential autophagy gene ATG5 revealed that ACER2-induced autophagy facilitates its effect on apoptosis. PMID:28294157
results indicate that LGASC of the breast is a low-grade triple-negative breast cancer that harbours a basal-like phenotype with no androgen receptor expression, and shows a high rate of PIK3CA mutations but no TP53 mutations PMID:29537649
this study shows an inhibitory effect of wild-type P53 gene transfer on graft coronary artery disease in rat model PMID:29425775
Our results suggest that TP53 c.215G>C, p. (Arg72Pro) polymorphism may be considered as a genetic marker for predisposition to breast cancer in Moroccan population PMID:29949804
higher level of the p53 isoform, p53beta, predict better prognosis in patients with renal cell carcinoma through enhancing apoptosis in tumors. PMID:29346503
TP53 mutations are associated with colorectal liver metastases. PMID:29937183
High expression of TP53 is associated with oral epithelial dysplasia and oral squamous cell carcinoma. PMID:29893337

Hide All

Subcellular Location

Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.; [Isoform 1]: Nucleus. Cytoplasm. Note=Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4.; [Isoform 2]: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm.; [Isoform 3]: Nucleus. Cytoplasm. Note=Localized in the nucleus in most cells but found in the cytoplasm in some cells.; [Isoform 4]: Nucleus. Cytoplasm. Note=Predominantly nuclear but translocates to the cytoplasm following cell stress.; [Isoform 7]: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm.; [Isoform 8]: Nucleus. Cytoplasm. Note=Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli.; [Isoform 9]: Cytoplasm.

Protein Families

P53 family

Tissue Specificity

Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3

Database Links

HGNC: 11998

UNIGENE: Hs.437460

KEGG: hsa:7157

STRING: 9606.ENSP00000269305

OMIM: 133239

Promotions