Human osteocrin,Ostn ELISA kit

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Name: Human osteocrin,Ostn ELISA kit
Brand: CUSABIO
SKU: CSB-E12021h

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Code: CSB-E12021h

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.312 ng/mL-20 ng/mL

Sensitivity: 0.078 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

OSTN ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit

Abbreviation

OSTN

Uniprot NO.

P61366

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Stem Cells

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Ostn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	99
	Range %	94-103
1:4	Average %	96
	Range %	92-99
1:8	Average %	95
	Range %	88-98

Recovery

The recovery of human Ostn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	97	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.552	2.699	2.626	2.510
10	2.254	2.373	2.314	2.198
5	1.804	1.928	1.866	1.750
2.5	0.889	0.926	0.908	0.792
1.25	0.517	0.498	0.508	0.392
0.625	0.320	0.304	0.312	0.196
0.312	0.216	0.202	0.209	0.093
0	0.118	0.114	0.116

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Human OSTN ELISA Kit was designed for the quantitative measurement of Human OSTN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Hormone that acts as a regulator of dendritic growth in the developing cerebral cortex in response to sensory experience. Induced in the brain following membrane depolarization and inhibits dendritic branching in neurons of the developing cortex. Probably acts by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production.

Gene References into Functions

The circulating concentration of musclin was significantly increased in type 2 diabetes mellitus patients. Results suggest that musclin has a strong relationship with insulin resistance in type 2 diabetes mellitus. PMID:28185530
findings suggest that, in response to sensory input, OSTN regulates features of neuronal structure and function that are unique to primates PMID:27830782
osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator PMID:14523025
Foxo1 downregulates musclin mRNA expression both in vitro and in vivo, which should explain insulin-mediated upregulation of this gene in muscle cells. PMID:17950246
Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID:17951249
Observational study of gene-disease association. (HuGE Navigator) PMID:19453261

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Subcellular Location

Secreted.

Protein Families

Osteocrin family

Tissue Specificity

Enriched in neocortical regions of the developing cerebral cortex. Not expressed in other compartments of the neocortical wall or in brain regions such as the hippocampus, striatum, mediodorsal nucleus of the thalamus and cerebellum. Also expressed in bon

Database Links

HGNC: 29961

UNIGENE: Hs.526794

KEGG: hsa:344901

STRING: 9606.ENSP00000342356

OMIM: 610280

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