* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human MPO antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- One vials standard (10 x concentrate) 1 x 200 μl
- --- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- HRP-conjugated MPO antibody (100 x concentrate) 1 x 120 μl --- Binds to the MPO derived from the sample, and HRP catalyzes the TMB substrate and thus changes the solution color.
- HRP-conjugate Diluent 1 x 20 ml ---Dilute the Biotin-antibody solution.
- Sample Diluent 2 x 20 ml ---Dilute the sample to an appropriate working concentration.
- Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
- TMB Substrate 1 x 10 ml --- The TMB-HRP coproduct appears blue after adding the chromogenic agent. After the enzyme reaction is terminated, the TMB product changes from blue to yellow. It can be quantified in the colorimeter.
- Stop Solution 1 x 10 ml --- Stop the color reaction.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
