Human Retinol binding protein 4,RBP-4 ELISA Kit

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Target Background

Name: Human Retinol binding protein 4,RBP-4 ELISA Kit
Brand: CUSABIO
SKU: CSB-E09423h

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Code: CSB-E09423h

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Citation （12）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 ng/mL-1000 ng/mL

Sensitivity: 3.9 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Target Name

retinol binding protein 4, plasma

Alternative Names

OTTHUMP00000020114 ELISA Kit; OTTHUMP00000020115 ELISA Kit; OTTHUMP00000020116 ELISA Kit; Plasma retinol binding protein 4 ELISA Kit; Plasma retinol-binding protein ELISA Kit; Plasma retinol-binding protein(1-176) ELISA Kit; PRBP ELISA Kit; PRO2222 ELISA Kit; RBP ELISA Kit; RBP4 ELISA Kit; RDCCAS ELISA Kit; RET4_HUMAN ELISA Kit; Retinol binding protein 4 ELISA Kit; Retinol binding protein 4 interstitial ELISA Kit; Retinol binding protein 4 plasma ELISA Kit

Abbreviation

RBP4

Uniprot NO.

P02753

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 ng/mL-1000 ng/mL

Sensitivity

3.9 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-98
1:200	Average %	98
	Range %	93-104
1:400	Average %	84
	Range %	82-88
1:800	Average %	90
	Range %	85-97

Recovery

The recovery of human RBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	96	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.634	2.702	2.668	2.566
500	2.234	2.217	2.226	2.124
250	1.496	1.527	1.512	1.410
125	0.856	0.879	0.868	0.766
62.5	0.446	0.468	0.457	0.355
31.2	0.267	0.285	0.276	0.174
15.6	0.206	0.212	0.209	0.107
0	0.105	0.099	0.102

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

Retinol binding protein 4 (RBP4) is a small plasma protein that serves as the primary carrier for retinol (vitamin A) in the bloodstream. This 21 kDa protein is synthesized primarily in the liver and forms a complex with transthyretin to transport retinol from hepatic stores to peripheral tissues. RBP4 has emerged as an important biomarker in metabolic research. Elevated levels have been linked with insulin resistance, type 2 diabetes, and cardiovascular disease, making it a valuable target for clinical and research studies.

The Human Retinol binding protein 4, RBP-4 ELISA Kit (CSB-E09423h) is designed for quantitative measurement of RBP4 in human samples. This sandwich ELISA works with serum, plasma, and tissue homogenates with a detection range of 15.6 ng/mL to 1000 ng/mL and sensitivity of 3.9 ng/mL. The assay requires 50-100 μL sample volume, operates with detection at 450 nm wavelength, and can be completed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in clinical research studies to quantify retinol binding protein 4 levels in human serum samples. The applications span cardiovascular disease research, metabolic studies, and proteomic validation experiments where RBP4 serves as a biomarker of interest.

• Cardiovascular research: Studies of RBP4 as a biomarker linking insulin resistance and coronary artery disease severity in patient populations
• Proteomic validation: Confirmation and quantification of candidate proteins identified through discovery-phase proteomic analyses in clinical samples
• Exercise intervention studies: Measurement of RBP4 levels alongside other metabolic and inflammatory markers in response to physical training protocols
• Biomarker profiling: Multi-analyte panels examining RBP4 in conjunction with adipokines, inflammatory cytokines, and acute-phase proteins in serum samples

■ Customer Reviews

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■ Q&A

I am interested in 52 of ELISA Kit CSB-E09423h. However, I may not order since the kit has not been tested with Urine samples. Is it possible arrange to have this kit tested with urine?

Thanks for your inquiry.
We tested urine samples before, but the tested samples are not many. We tested 10 samples before and the test value is 15--60ng/ml.We suggest you order one sample kit and do a pretest first before big purchase.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.

Gene References into Functions

Baseline RBP4 levels and MELD scores predicted 21-day (</=10 mg/L) and 1-year (>/=25) mortality, respectively. In critically ill patients with underlying liver disease, with a link to eGFRs, INRs and TC levels, the baseline RBP4 may serve as a marker for short-term mortality. PMID:28588245
our study provides clinical evidence revealing that the serum concentrations of RBP4 were elevated in NAFLD patients in a Chinese population. These findings indicated that RBP4 might be a noninvasive molecular biomarker that detects the presence of NAFLD in middle-aged and elderly population. PMID:28332619
Vitreous levels of APOA1 and RBP4 in human rhegmatogenous retinal detachment associated with choroidal detachment reflects the severity of disease. PMID:29618920
The overexpression of RBP4 increased cell proliferation, whereas siRNA-mediated RBP4 knockdown significantly decreased HTR8/SVneo cell proliferation via activation of PI3K/AKT signaling. PMID:30015949
High RBP4 expression is associated with hypertriglyceridemia. PMID:29747616
We observed that knockdown of RBP4 can greatly suppress ovarian cancer cell migration and proliferation. PMID:29642915
This study showed that the Levels of circulating RBP4 was significantly higher in Chronic Kidney Disease than in control. PMID:29678848
Our results demonstrate that IR is associated with high circulating RBP4 and that suppressed RBP4 adipose tissue expression is accompanied by reduced GLUT4 expression in HD. Renal transplantation or HDF are effective in lowering serum RBP4 levels. PMID:29333450
Data suggest that serum levels of RBP4 and LGAL3BP are up-regulated after menopause when complicated by NAFLD (non-alcoholic fatty liver disease); RBP4 and LGAL3BP may serve as biomarkers of NAFLD in postmenopausal women. (RBP4 = retinol binding protein 4; LGAL3BP = galectin-3 binding protein) PMID:29679552
Elevated levels of RBP4 were not associated with an increased risk of ischemic stroke among women. PMID:28888344
Study showed that dietary weight loss-induced changes in angiotensin-converting enzyme activity, free fatty acids and RBP4 independently contribute to weight regain prediction. PMID:29122953
RBP4 is involved in all-trans retinoic acid-induced cleft palate. PMID:28849085
the 1.5A resolution structures of human holo-RBP4 and of the protein saturated with palmitic and lauric acid and discuss the interaction of the fatty acids and retinol with the protein, are reported. PMID:29414511
Circulating RBP4 levels may not be associated with nonalcoholic fatty liver disease. PMID:28931435
Plasma PEDF and RBP4 identified insulin resistance in subjects with no prior diagnosis of diabetes. PMID:28648555
Serum RBP4 level was significantly higher and closely associated with blood pressure in prehypertensive Chinese. PMID:28639612
Conclusion RBP4 may be used as a predictive factor of diabetic nephropathy patients complicated with silent cerebral infarction (SCI) and is positively correlated with cognitive dysfunction. RBP4/Lp-PLA2/Netrin-1 pathway activation may be one of the occurrence mechanisms in diabetic nephropathy complicated with SCI. PMID:28704853
these data demonstrate a key role of STRA6 and RBP4 in the maintenance of colon cancer self-renewal and that this pathway is an important link through which consumption of HFD contributes to colon carcinogenesis. PMID:28689994
Data suggest that, in subjects with chronic kidney disease, renal function is inversely related to serum RBP4 levels; as GFR decreases, the relationship between RBP4 and insulin resistance is attenuated. (GFR = glomerular filtration rate, a measure of kidney function) PMID:28473187
Results identified RBP4 as a promising acute liver failure (ALF) biomarker among energy metabolism-related proteins. RBP4 values signaled liver injury at an early stage, as its decreasing level was observed before ALT elevation in the pig ALF model, and its significant decreasing level in ALF patients. PMID:28336726
Elevated RBP4 is detectable early in pregnancy and has strong correlation with preeclampsia and premature birth. PMID:28338737
RBP4-induced inflammation is largely mediated by TLR4. PMID:28400700
RBP4 may be associated with higher cardiovascular risk in overweight/obese adolescent girls, but this association is mediated by abdominal obesity. PMID:29120147
Bi-allelic mutations in RBP4 were identified (c.248+1G>A), consistent with a diagnosis of inherited vitamin A deficiency. PMID:27892788
Childhood obesity may be associated with variations in RBP4 gene. The presence of selective SNPs in the RBP4 gene may account for metabolic complications. PMID:26611784
Low levels of vitamin A, E and RBP4 at the time of renal cell carcinoma diagnosis are associated with a poorer prognosis after surgery. PMID:28668878
RBP4 is some 6-fold lower when active TB patients have chronic energy deficiency (CED) than when BMI is >25 kg/m sq; however, free fatty acids were not associated with CED in active TB patients, which may be a type 2 error or represent an energy impasse where infection and the host's metabolic needs are in competition. PMID:28625041
Data show that the tissue-specific expression pattern of human transgenic retinol binding protein 4 (hRBP4orf) was roughly the same as that of mouse Rbp4. PMID:28134916
Elevated levels of RBP4 are associated with higher cardiovascular mortality among men with type 2 diabetes. PMID:27609367
RBP4 and retinol levels were increased approximately twofold in patients with chronic kidney disease. PMID:27277845
RBP4 level was associated with increased arterial stiffness in young subjects with family history of type 2 diabetes. PMID:26868132
Retinol-binding protein 4 is able to function as biomarker to distinguish severe pre-eclampsia from normal pregnancy. More importantly, these results may shed light on the role of Retinol-binding protein 4 in the pathogenesis in pre-eclampsia. PMID:27279411
High level of RBP4 is associated with obesity. PMID:26794633
The positive association between circulating RBP4 and ApoB-containing lipoproteins in a steady metabolic state, as well as during a hypocaloric diet, appears to be attenuated in patients with very high serum triglycerides. PMID:27086684
Urinary KNG1 and RBP4 clearly respond to AKI. Additionally, RBP4 could predict recovery by monitoring this protein levels over time after AKI, as RBP4 reflects patient's normalization earlier than sCr values do. PMID:26792617
SNPsRBP4 was associated with age at onset in familial amyloid polyneuropathy. PMID:26286643
Serum RBP4 is negatively related to estrogen in Chinese women with obesity. PMID:26960804
Dietary vitamin A may reduce abdominal adiposity and promote visceral to subcutaneous body fat redistribution during adolescence in an RBP4-dependent manner. PMID:26667887
The results of this meta-analysis support the hypothesis that RBP4 is a modest independent risk factor for gestational diabetes mellitus (i.e., nonobese patients with gestational diabetes mellitus might express RBP4 at abnormal levels).The association between RBP4 rs3758539 polymorphism and gestational diabetes mellitus risk was not confirmed. PMID:26975349
RBP-4 levels are higher in patients with clinical hypothyroidism and exhibit a marked decrease after normalization of thyroid function in both hyper and hypothyroid patients. We suggest that RBP-4 may play a role in the metabolic disturbances which accompany thyroid dysfunction. PMID:26575118
Fasting concentrations of RBP-4 were negatively correlated with BMI and waist-hip ratio, whereas lipocalin-2 levels were positively associated with BMI and waist-hip ratio. PMID:26069091
male mice are susceptible to high fat diet-induced hyperglycaemia and display higher plasma RBP4 levels, possibly due to its over-expression in visceral adipose depots PMID:26619134
RBP4 concentrations were significantly lower in hepatitis C subjects compared to controls. PMID:26927700
Presence of gestational diabetes mellitus risk factors does not have an impact on early second trimester RBP-4 values in pregnant subjects. PMID:25227411
This study describe the discovery and subsequent replications of RBP4 and its combination with circulating GFAP as plasmatic biomarkers for hyperacute stroke subtype differentiation. PMID:26526443
Gc1s/Gc1s phenotype variant of DBP and the unbound fraction of plasma RBP4 may be considered as factors related with the incidence, and possibly the risk, of IR in CHC patients. PMID:26962819
RBP correlated with IgG4 and IgA. RBP also correlated positively with age and with influenza virus-specific antibody neutralization titres. PMID:26425827
Genetic variants of retinol-binding protein 4 in adolescents are associated with liver function and inflammatory markers but not with obesity and insulin resistance PMID:26440092
Baseline serum RBP4 levels were independently associated with incident diabetes in Asian Indian men with impaired glucose tolerance. PMID:25810022
Findings suggested that Asian women with gestational diabetes mellitus had increased circulating RBP4 levels in their second/third trimester. [Meta-Analysis] PMID:25703255