Human Mast Cell Carboxypeptidase ELISA Kit

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Name: Human Mast Cell Carboxypeptidase ELISA Kit
Brand: CUSABIO
SKU: CSB-E13756h

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Code: CSB-E13756h

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.312 ng/mL-20 ng/mL

Sensitivity: 0.078 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Target Name

carboxypeptidase A3 (mast cell)

Alternative Names

carboxypeptidase A ELISA Kit; carboxypeptidase A3 (mast cell) ELISA Kit; Carboxypeptidase A3 ELISA Kit; carboxypeptidase A3 mast cell ELISA Kit; CBPA3_HUMAN ELISA Kit; Cpa3 ELISA Kit; Mast cell carboxypeptidase A ELISA Kit; mast cell carboxypeptidase A3 ELISA Kit; MC CPA ELISA Kit; MC-CPA ELISA Kit; tissue carboxypeptidase A ELISA Kit

Abbreviation

CPA3

Uniprot NO.

P15088

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	94-103
1:4	Average %	97
	Range %	94-100
1:8	Average %	94
	Range %	86-98

Recovery

The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	94-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.477	2.453	2.465	2.366
10	1.993	1.924	1.959	1.860
5	1.280	1.271	1.276	1.177
2.5	0.685	0.678	0.682	0.583
1.25	0.433	0.424	0.429	0.330
0.625	0.286	0.269	0.278	0.179
0.312	0.190	0.179	0.185	0.086
0	0.101	0.097	0.099

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Human CPA3 ELISA Kit was designed for the quantitative measurement of Human CPA3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Gene References into Functions

Carboxypeptidase A3 was differentially expressed between Fibromyalgia patients and healthy controls. PMID:27157394
Urine CPA3 was increased in patients with chronic prostatitis/chronic pelvic pain syndrome compared to controls. PMID:24726923
The cDNA encoding human colon mast cell carboxypeptidase can be successfully cloned and expressed in E. coli and P. pastoris. PMID:14760754
Role in regulating innate immunity responses and regulating secretory granule homeostasis PMID:19643669