Human Interleukin 26,IL-26 ELISA Kit

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Name: Human Interleukin 26,IL-26 ELISA Kit
Brand: CUSABIO
SKU: CSB-E11716h

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Code: CSB-E11716h

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Citation （11）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, urine, tissue homogenates, cell lysates, cell culture supernates

Detect Range: 62.5 pg/mL-4000 pg/mL

Sensitivity: 15.6 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

AK155 ELISA Kit; IL 26 ELISA Kit; IL-26 ELISA Kit; IL26 ELISA Kit; IL26_HUMAN ELISA Kit; Interleukin 26 ELISA Kit; Interleukin-26 ELISA Kit; Protein AK155 ELISA Kit

Abbreviation

IL26

Uniprot NO.

Q9NPH9

Species

Homo sapiens (Human)

Sample Type

serum, urine, tissue homogenates, cell lysates, cell culture supernates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-26 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	100
	Range %	95-106
1:8	Average %	97
	Range %	92-104

Recovery

The recovery of human IL-26 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	102	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.749	2.798	2.774	2.616
2000	2.315	2.364	2.340	2.182
1000	1.843	1.889	1.866	1.708
500	1.399	1.381	1.390	1.232
250	0.899	0.888	0.894	0.736
125	0.483	0.487	0.485	0.327
62.5	0.316	0.329	0.323	0.165
0	0.157	0.159	0.158

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Human Interleukin 26,IL-26 ELISA Kit

Description

This Human IL26 ELISA Kit was designed for the quantitative measurement of Human IL26 protein in serum, urine, tissue homogenates, cell lysates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Regulation of Pulmonary Graft-versus-Host Disease by IL-26+ CD26+ CD4 T Lymphocytes Ohnuma K et al,/,2015
The cytokine interleukin-26 as a biomarker in pediatric asthma Konradsen JR.et al,/,2016
IL-1β Induces the Rapid Secretion of the Antimicrobial Protein IL-26 from Th17 Cells Weiss DI, et al,/,2019
Interleukin-26 A cytokine at the interface between innate and adaptive immunity HC Hawerkamp,/,2020
Single-cell atlas of colonic CD8+ T cells in ulcerative colitis D Corridoni,/,2020
Involvement of IL-26 in bronchiolitis obliterans syndrome but not in acute rejection after lung transplantation JM Magnusson,/,2022
Differentiation of IL-26+ TH17 intermediates into IL-17A producers via epithelial crosstalk in psoriasis A Fries,/,2023
Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen MA Kovach,/,2023
Human neutrophils drive skin autoinflammation by releasing interleukin (IL)-26 A Baldo,/,2024
Systemic increase in IL-26 is associated with severe COVID-19 and comorbid obstructive lung disease EI Cardenas, J Robertson, S Misaghian,/,2024
Increased IL-26 associates with markers of hyperinflammation and tissue damage in patients with acute COVID-19 EI Cardenas, S Ekstedt, K Piersiala, M Petro, A Karlsson, Å Kågedal, S Kumlien Georén,/,2022

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

May play a role in local mechanisms of mucosal immunity and seems to have a proinflammatory function. May play a role in inflammatory bowel disease. Activates STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN and AKT. Induces expression of SOCS3, TNF-alpha and IL-8, secretion of IL-8 and IL-10 and surface expression of ICAM1. Decreases proliferation of intestinal epithelial cells. Is inhibited by heparin.

Gene References into Functions

this paper shows that IL-26 is overexpressed in Behcet's disease and enhances Th17 related -cytokines PMID:28811236
The varIL26 genotype is associated with reduced PMN capacity to kill bacteria. A varIL26 genotype is associated with decreased levels of anti-TNF-alpha in CD patients. IL26 may help explain the role of bactDNA as a risk factor of flare in CD patients. PMID:28879509
IL-26 activates STAT1/3 and leads to the induction of IL-6 and IL-8 expression in non-transformed cells derived from human colon. PMID:28852311
IL-26 levels are higher in synovial fluid compared to plasma in spondyloarthritis. IL-26 was identified in axial facet joints of spondyloarthritis patients. Myofibroblasts from the spondyloarthritis synovium produce large amounts of IL-26. IL-26 induces bone mineralization in human osteoblasts. PMID:28365787
this study shows that IL-26 is a unique cationic protein more similar to a soluble pattern recognition receptor than to conventional cytokines PMID:28356384
this study shows that single nucleotide polymorphism near IL-26 gene is associated with familial vitiligo and existence of halo nevi in Estonian patients PMID:26429320
Our study indicates that IL-26 is a potential biomarker of disease severity in pediatric asthma without signs of Th2-mediated inflammation. PMID:27029915
IL-26 is emerging as a potentially important player in host defense and may also be a pathogenic factor in the chronic inflammatory disorders of humans. [Review] PMID:26202572
The negative influence of IL-26 on the anti-mycobacterial activity and its constitutive presence in both serum and monocyte supernatants prompt a proposal that IL26 as a candidate gene for tuberculosis susceptibility. PMID:25157980
IL-26 differentially modulates the infection by different enveloped viruses. PMID:23875025
Elevated levels of IL-26 in human gastric cancer promote proliferation and survival by modulating STAT1/STAT3 signaling. PMID:23704922
IL-26 appears as a novel proinflammatory cytokine, located upstream of the proinflammatory cascade, that may constitute a promising target to treat rheumatoid arthritis and chronic inflammatory disorders. PMID:23055831
Identify a second enhancer element positioned between IL26 and IFNG required for both IL26 and IFNG expression. One function of this enhancer is to facilitate recruitment of RNA polymerase II to promoters of both genes. PMID:22622197
common polymorphisms in the IFNgamma/IL-26 gene region may contribute to sex bias in susceptibility to rheumatoid arthritis, by distorting the propensity of female carriers versus male carriers to contract this disease. PMID:14558082
The active receptor complex for IL-26 is a heterodimer composed of two receptor proteins: IL-20 receptor 1 and IL-10 receptor 2. Signaling through the IL-26 receptor results in activation of STAT1 and STAT3. PMID:14764663
sensitivity to recombinant interleukin-26(IL-26) of various cell lines strictly correlated with the expression of IL-20 receptor 1 and blocking antibodies against either IL-10 receptor 2 or IL-20 receptor 1 inhibited IL-26-dependent signal transduction PMID:15178681

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Subcellular Location

Secreted.

Protein Families

IL-10 family

Tissue Specificity

Expressed in HVS transformed T-cells but not other T-cell lines or primary stimulated T-cells. Expressed in colonic T-cells including Th17 inflammatory T-cells; the expression is significantly increased in serum of patients with Crohn's disease (at protei

Database Links

HGNC: 17119

UNIGENE: Hs.272350

KEGG: hsa:55801

STRING: 9606.ENSP00000229134

OMIM: 605679

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