Human Interferon epsilon(IFNE) ELISA kit

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Target Background

Name: Human Interferon epsilon(IFNE) ELISA kit
Brand: CUSABIO
SKU: CSB-EL011049HU

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Code: CSB-EL011049HU

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Target Name

interferon, epsilon

Alternative Names

IFNE ELISA Kit; IFNE1 ELISA Kit; UNQ360/PRO655 ELISA Kit; Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit

Abbreviation

IFNE

Uniprot NO.

Q86WN2

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-95
1:2	Average %	93
	Range %	88-99
1:4	Average %	92
	Range %	87-100
1:8	Average %	95
	Range %	88-99

Recovery

The recovery of human IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	93	88-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.058	2.011	2.035	1.931
500	1.664	1.547	1.606	1.502
250	1.188	1.149	1.169	1.065
125	0.739	0.704	0.722	0.618
62.5	0.561	0.521	0.541	0.437
31.2	0.375	0.354	0.365	0.261
15.6	0.219	0.211	0.215	0.111
0	0.105	0.103	0.104

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Human Interferon epsilon(IFNE) ELISA kit

Description

This Human IFNE ELISA Kit was designed for the quantitative measurement of Human IFNE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

■ Customer Reviews

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Defining a Role for Interferon Epsilon in Normal and Complicated Pregnancies D Miller,/,2022

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral and bacterial genital infections.

Gene References into Functions

IFN epsilon protects macrophages against HIV infection through a type I IFN independent mechanism. PMID:27942584
human IFNvarepsilon suppresses HIV replication at multiple stages of infection. PMID:28045025
female sex workers decreased susceptibility to HIV-1 infection also have increased levels of IFNE gene and protein expression in the cervical epithelium PMID:26555708
T Allele of nonsense polymorphism (rs2039381, Gln71Stop) of interferon-epsilon is a risk factor for the development of intracerebral hemorrhage. PMID:24055696
Full-length IFN-epsilon 5'UTR markedly suppressed mRNA expression under basal conditions. It contains 2 stable stem-loop structures which associate with importin 9. Loop 1 is essential for regulation of mRNA expression. PMID:23851686
genetic polymorphism is related to onset time of vitiligo in Korean patients PMID:23802172
Observational study of gene-disease association. (HuGE Navigator) PMID:20574843
Observational study of gene-disease association. (HuGE Navigator) PMID:20588308
Observational study of gene-disease association. (HuGE Navigator) PMID:20601674
The structue and mRNA expression pattern of IFN-epsilon1 suggest that it may have a function distinct from those other members of type I INF. PMID:15233997
TNF-alpha leads to stabilization of IFN-epsilon mRNA, increased IFN-epsilon synthesis, engagement of type I IFNRs, increased STAT1 expression and phosphorylation, and up-regulation of retinoic acid-inducible gene-I expression PMID:17878351
A meta-analysis and a single-nucleotide polymorphism (SNP) rs1333049 representing the 9p21.3 locus provide unprecedented evidence for association between genetic variants at chromosome 9p21.3 and risk of coronary artery disease. PMID:18362232
In macrophages IFN-tau increased the synthesis of 2',5'-oligoadenylate synthetase/RNase L, MxA protein, IL-10 & IL-6, but not of IL-1ss or TNF-alpha. PMID:18842358