Human E-Selectin ELISA kit

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Code: CSB-E04540h

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.312 ng/mL-20 ng/mL

Sensitivity: 0.078 ng/mL

Trial Size:

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Target Background

Product Details

Alternative Names

SELE; ELAM1; E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD antigen CD62E

Abbreviation

SELE

Uniprot NO.

P16581

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	2.382	2.629	2.463	2.564	2.592	2.546
SD	0.041	0.043	0.036	0.049	0.052	0.047
CV(%)	5.76	5.61	4.93	6.52	6.86	6.28

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human E-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

95-110

1:2

Average %

Range %

91-103

1:4

Average %

Range %

82-92

1:8

Average %

Range %

89-98

Recovery

The recovery of human E-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
Heparin plasma (n=4)	98	90-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.370

2.274

2.322

2.154

1.859

1.779

1.819

1.651

1.229

1.209

1.219

1.051

2.5

0.740

0.734

0.737

0.569

1.25

0.437

0.431

0.434

0.266

0.625

0.347

0.339

0.343

0.175

0.312

0.207

0.204

0.206

0.038

0.168

0.167

0.168

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

E-selectin (SELE) is a cell adhesion molecule primarily expressed on activated endothelial cells in response to inflammatory stimuli such as cytokines and bacterial endotoxins. This glycoprotein plays an important role in the initial steps of leukocyte recruitment during inflammation by mediating the rolling and tethering of neutrophils, monocytes, and certain T-cell subsets along the vascular endothelium. E-selectin expression is rapidly upregulated during acute inflammatory responses. It serves as an important biomarker for endothelial activation and vascular inflammation in various pathological conditions.

The Human E-Selectin ELISA kit (CSB-E04540h) is a quantitative sandwich immunoassay designed for measuring selectin E levels in human serum, plasma, and tissue homogenates. The assay offers a detection range of 0.312 ng/mL to 20 ng/mL with a sensitivity of 0.078 ng/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours, with optical density measurements taken at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in clinical research studies to measure E-selectin levels in human biological samples as part of biomarker profiling. The kit supports research into vascular inflammation and endothelial dysfunction across various disease contexts. Researchers have used this assay alongside other inflammatory and vascular markers to measure disease-related changes in circulating biomarker levels.

• Vascular inflammation research: Measuring E-selectin as a marker of endothelial activation in serum samples from patients undergoing clinical evaluation
• Multi-biomarker profiling: Integration with panels measuring various inflammatory mediators, adhesion molecules, and vascular markers to provide disease characterization
• Clinical biomarker studies: Measurement of circulating E-selectin levels in plasma samples as part of broader research into inflammatory and vascular pathways
• Pre-surgical screening: Evaluation of E-selectin concentrations in patient serum samples collected prior to surgical procedures as part of biomarker screening protocols

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Citations

Chemical constituents and sources of ambient particulate air pollution and biomarkers of endothelial function in a panel of healthy adults in Beijing, China. Wu S.et al,/,2016
MicroRNA-1185 Promotes Arterial Stiffness though Modulating VCAM-1 and E-Selectin Expression Deng H.et al,/,2017
The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium Layne K.et al,/,2017
Predictive value of combined LIPS and ANG-2 in critically ill patients with ARDS risk factors Zhi Xu.et al,/,/
Predictive Value of Combined LIPS and ANG-2 Level in Critically Ill Patients with ARDS Risk Factors Zhi Xu.et al,/,2018
Pomegranate Juice Increases Sirtuin1 Protein in Peripheral Blood Mononuclear Cell from Patients with Type 2 Diabetes: A Randomized Placebo Controlled Clinical Trial Sohrab Golbon.et al,/,2018
Effect of Radiation on the Expression of CVD-related miRNAs, Inflammation and Endothelial Dysfunction of HUVECs Esplugas R, et al,/,2019
EXPANSION, ACTIVATION AND FUNCTION PROMOTION OF MYELOID-DERIVED SUPPRESSOR CELLS IN ADJUVANT-INDUCED ARTHRITIS Abd-Elhalem S S, et al,/,2019
Higher serum PGE2 is a predicative biomarker for postoperative delirium following elective orthopedic surgery in elderly patients M Mao,/,2022
MiR-221-3p improves the prognostic value of lung ultrasound score in acute respiratory distress syndrome and modulates the inflammatory response D Liu, D Yang, Y Zhang, Y Wang, H Yu,/,2025

Target Background

Function(From Uniprot)

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Gene References into Functions

Results highlight novel mechanisms by which p38 downregulates the expression of E-selectin through miR-146a and miR-31 following inflammatory stimuli associated to cancer progression. PMID:29402939
Levels of E and P selectin showed positive correlation with the length of stay. PMID:29517629
Our study shows a significant association of E-selectin gene polymorphism(Leu554Phe) with essential hypertension. PMID:29178542
The highest levels of sL- and sE-selectin were observed in colorectal patients with lymph node metastasis. PMID:27775438
Circulating CD62E(+) microparticle levels are abnormal in subjects with pre-diabetes. PMID:28927403
sVCAM-1 reflects xerostomia in primary Sjogren's syndrome. sICAM-1 and sE-selectin may be additional parameters of secondary Sjogren's syndrome activity. PMID:29068581
Neutrophil rolling over E-selectin at precise shear stress transmits tension and catch-bond formation with L-selectin via sLe(x), resulting in focal clusters that deliver a distinct signal to upshift beta2-integrins to a high-affinity state. Rivipansel effectively blocked formation of selectin catch-bonds, revealing a novel mechanotransduction circuit PMID:28811304
E-selectin-dependent rolling promotes adhesion to HA by both physically slowing cells and enabling them to position proximal to the surface, thereby increasing the on rate of adhesion. PMID:28765175
High E-selectin expression is associated with breast cancer metastasis. PMID:27220365
his study shows that two adhesion molecules, shed as soluble forms, are elevated during the acute phase of leptospirosis: E-selectin and s-ICAM1. These molecules may interfere with the process of immune cell recruitment to clear Leptospira at tissue levels. PMID:28686648
ELAM-1 upregulation may promote disorder in adhesion, and migration of the cells leading to the endometriosis disorder. PMID:27433816
Elevated endothelium-related mediators (vWF, E-selectin and EPCR) appear to participate in the development of pancreatic necrosis and may be a potential indicator of overall prognosis. PMID:28007459
interplay between flow-dependent on-rate and off-rate of E-selectin-ligand bonds determine flow-enhanced cell rolling stability PMID:27558713
High SELE expression is associated with Inflamed Atherosclerotic Plaque. PMID:27237221
In this study, we addressed this question by analysing sLex expression together with two glycoproteins (BST-2 and LGALS3BP), shown to interact with E-selectin in a carbohydrate-dependent manner, in a cohort of 249 invasive breast cancers PMID:27176937
SELE expression is significantly upregulated in human masticatory mucosa during wound healing PMID:28005267
E-selectin concentrations in this study were associated with hyperglycemia, possibly reflecting early endothelial damage. E-selectin was not useful to assess carotid intima-media thickness, a marker of subclinical atherosclerosis, which appeared to be determined by aging and male gender. PMID:27141013
Plasma E-selectin is overexpressed in pediatric patients with mycoplasma pneumoniae pneumonia and myocardial injury. PMID:26843511
The significant presence of CLA+ T cells and E-selectin (CD62E) expressions in the OLPG suggests their involvement in the etiopathogenesis of oral lichen planus ; however, only a weak correlation between CLA+ T cells and E-selectin was observed. PMID:26332489
BD patients have a lower expression of ICAM-1, VCAM-1, and E-selectin in their tumor tissues. BD-HDL facilitates the adhesion of tumor cells to vascular endothelium by upregulating the expression of ICAM-1 and VCAM-1, thereby promoting the initial progression of breast cancer metastasis PMID:26872904
The E-selectin S149R polymorphisms is associated with the oncogenesis of breast cancer. PMID:26828984
Neisseria meningitidis caused a high level of E-selectin expression elicited by the activity of phosphorylated ATF2 transcription factor on the E-selectin promoter. PMID:26153406
E-selectin is more effective than cTnI in diagnosing myocardial injury in children. PMID:26617843
Inhibition of E-selectin expression allows an anti-tumoral effect on sLex-expressing hepatocellular carcinoma tumors in vivo PMID:26988221
Glycosphingolipids likely contribute to human myeloid cell adhesion to E-selectin under fluid shear, particularly the transition of rolling cells to firm arrest. PMID:26868209
Data show that both mucin16 (MUC16) and podocalyxin (PODXL)-E-selectin-mediated interactions are mechanically stronger than like L-selectin interactions at the single-molecule level. PMID:26329844
These findings provide consistent evidence that A516C and G98T polymorphisms of the SELE gene may be associated with increased susceptibility of coronary artery disease. PMID:26937892
Glaucomatous MYOC mutations activate the IL-1beta/NF-kappaB inflammatory stress response and the glaucoma marker SELE in trabecular meshwork cells. PMID:26396484
P. gingivalis was shown to activate NOD1, NOD2, and TLR2 expression, resulting in increased E-selectin expression in endothelial cells; direct inhibition of NF-kappaB and P38 MAPK significantly attenuated E-selectin expression induced by P. gingivalis in endothelial cells PMID:25939768
The number of E-selectin-positive cells was significantly higher in the tuberculoid form than in the lepromatous form of leprosy skin lesions. PMID:26051909
Gene-based study revealed significant associations of the SELE gene with Blood Pressure phenotypes for the first time among the Chinese Han population. PMID:25424718
This study showed that no significant differences in F206L Polymorphism of l-Selectin Between the Patients with Bronchial Asthma and Control. PMID:24798295
Blood ESEL was higher in pregnant women with preeclampsia, and non-pregnant women with a history of preeclampsia than women without preeclampsia or a history of preeclampsia. PMID:26116870
The findings suggest that C allele carriers of the SELE gene polymorphism (A561C) might be predisposed to EH in the Chinese population. PMID:25171839
E-selectin promoter was highly and specifically acetylated in the confluent monolayer after tumor necrosis factor alpha activation. PMID:24690766
E-selectin interactions with glycoprotein ligands (CD44/hematopoietic cell E-/L-selectin ligand and PSGL-1) mediate the initial capturing of cells out of flow. PMID:26124272
SELE rs3917412 and MTHFR rs1801133 SNPs could serve as pharmacogenetic predictors of tumor recurrence in patients with early-stage colon cancer treated with oxaliplatin-based adjuvant chemotherapy PMID:24980946
sE-selectin and the LDL were the most important predictors of late arteriovenous fistula stenosis PMID:24627030
The C allele of E-selectin A561C gene polymorphism might increase the essential hypertension (EH) risk in Asian population, whereas the T allele of E-selectin C1839T gene polymorphism might decrease the EH risk. [meta-analysis] PMID:25003340
Bereaved respondents had higher levels of inflammatory biomarkers IL-6 and sE-selectin, but not CRP and sICAM-I than the non-bereaved. PMID:24266503
SE-selectin and PAPPA may contribute to future cardiovascular events in women post preeclampsia; in contrast, ApoB may be involved in a protective mechanism PMID:25238218
the haplotype TCC in the Han hypertensive patients and the haplotype GGC in the Mongolian patients had independent effects in increasing the risk for essential hypertension; multiple single-nucleotide polymorphisms in combination in SELE may confer a risk of hypertension. PMID:25748737
Found the AC genotype to be a significant risk factor for hemorrhagic stroke and we also found significant differences in AC genotype and C allele among hemorrhagic stroke and ischemic stroke patients. PMID:25168603
The rs5361 E-selectin gene polymorphism is not a susceptibility marker for ACS in Western Mexico population. However, sE-selectin may be a biological marker of ACS. PMID:25147432
ST3Gal-4 is the primary sialyltransferase regulating the synthesis of E-, P-, and L-selectin ligands on human myeloid leukocytes PMID:25498912
an upregulation of E-selectin and the associated influx of neutrophils may play a significant role in the resolution of inflammation as well as for the pathophysiology of nasal polyps of Caucasian chronic rhinosinusitis patients. PMID:24995349
in human aortic and vein endothelial cells, expression and release of ICAM-1 and E-selectin were induced by treatment with conditioned medium collected from Pal-stimulated pTHP-1 cells PMID:25201247
CD62E was found to be a new biomarker for patients with hypertension and hyperlipidemia. PMID:24820735
High E-Selectin expression is associated with small cell lung cancer metastasis. PMID:24699516
there was association between the E-selectin S128R polymorphism and the risk of cancer. PMID:24815478