Human Chitinase-3-like protein 2(CHI3L2) ELISA kit

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Human Chitinase-3-like protein 2(CHI3L2) ELISA kit

Name: Human Chitinase-3-like protein 2(CHI3L2) ELISA kit
Brand: CUSABIO
SKU: CSB-EL005347HU

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Code: CSB-EL005347HU

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.78 ng/ml-50 ng/ml

Sensitivity: 0.19 ng/ml

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

CH3L2_HUMAN ELISA Kit; CHI3L2 ELISA Kit; Chitinase 3 like 2 ELISA Kit; Chitinase-3-like protein 2 ELISA Kit; Chondrocyte protein 39 ELISA Kit; OTTHUMP00000014028 ELISA Kit; YKL 39 ELISA Kit; YKL-39 ELISA Kit; YKL39 ELISA Kit

Abbreviation

CHI3L2

Uniprot NO.

Q15782

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.78 ng/ml-50 ng/ml

Sensitivity

0.19 ng/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Stem Cells

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CHI3L2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-91
1:2	Average %	101
	Range %	95-105
1:4	Average %	102
	Range %	98-109
1:8	Average %	96
	Range %	91-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.701

2.688

2.695

2.582

2.344

2.192

2.268

2.155

12.5

1.704

1.729

1.717

1.604

6.25

1.151

1.137

1.144

1.031

3.125

0.678

0.699

0.689

0.576

1.56

0.388

0.366

0.377

0.264

0.78

0.238

0.241

0.240

0.127

0.115

0.111

0.113

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

■ Customer Reviews

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Chitinase 3-like proteins as diagnostic and prognostic biomarkers of multiple sclerosis Hinsinger G et al,/,2015

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.

Gene References into Functions

This study shown that CHI3L2 is regulated differently in healthy and late onset Alzheimer"s disease brain depending on the sex. PMID:27653898
Structural analysis demonstrates that YKL-39 interacts with chitooligosaccharides through hydrogen bonds and hydrophobic interactions. Thermodynamic analysis indicates that binding of chitooligosaccharide to YKL-39 is mainly driven by enthalpy. PMID:25477513
These data suggest that hYKL-39 is a novel growth and differentiation factor involved in cartilage homeostasis. PMID:23291184
YKL-39 possesses a chitinase-like fold, but lacks key active-site residues required for catalysis. PMID:22742450
ERK1/2 phosphorylation by CHI3L2 inhibits cell mitogenesis and proliferation. PMID:22211103

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Subcellular Location

Secreted.

Protein Families

Glycosyl hydrolase 18 family

Tissue Specificity

Highest expression in chondrocytes, followed by synoviocytes, lung and heart. Not detected in spleen, pancreas, and liver. May also be expressed in developing brain and placenta.

Database Links

HGNC: 1933

UNIGENE: Hs.514840

KEGG: hsa:1117

STRING: 9606.ENSP00000358763

OMIM: 601526

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