Horse Interleukin 6(IL-6)ELISA Kit

Product Details

Customer Reviews and Q&A

Citations

Target Background

Name: Horse Interleukin 6(IL-6)ELISA Kit
Brand: CUSABIO
SKU: CSB-E16634Hs

CSB-E16634Hs

Rate （N/A）
Review （0）
Citation （6）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 3.12 pg/mL-200 pg/mL

Sensitivity: 0.78 pg/mL

Send an Inquiry

Start an on-line Chat

Orders can only be placed from within the U.S.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Alternative Names

IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit

Abbreviation

IL-6

Uniprot NO.

Q95181

Species

Equus caballus (Horse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-98
1:2	Average %	90
	Range %	85-95
1:4	Average %	96
	Range %	92-101
1:8	Average %	104
	Range %	96-108

Recovery

The recovery of horse IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-97
EDTA plasma (n=4)	95	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.267	2.235	2.251	2.136
100	1.482	1.589	1.536	1.421
50	0.954	0.999	0.977	0.862
25	0.602	0.612	0.607	0.492
12.5	0.432	0.452	0.442	0.327
6.25	0.269	0.289	0.279	0.164
3.12	0.218	0.230	0.224	0.109
0	0.111	0.119	0.115

Troubleshooting and FAQs

ELISA kit FAQs

Datasheet & COA

Please contact us to get it.

Description

This Horse IL-6 ELISA Kit was designed for the quantitative measurement of Horse IL-6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

■ Customer Reviews

Submit a Review here

Metabolic responses of acute phase protein, antioxidant and cytokine in Arabian Endurance horses L.Adamu et al,/,2013
OSTEOARTHRITIC SCLEROTIC SUBCHONDRAL OSTEOBLASTS SECRETED ELEVATED CONCENTRATION OF FIBULIN-3 FRAGMENTS IN VITRO LPS M.et al,/,2016
Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells Abdelhamid L.et al,/,2017
The use of a metabolic disorder index as a predictor for metabolic eliminations in endurance horses L Adamu.et al,/,2017
Validity of an established metabolic disorder index as a predictor for metabolic eliminations in endurance horses M.R. Nur Zul Izzati .et al,/,2018
Analgesic Effect of Dexmedetomidine-Nalbuphine Combination vs. Dexmedetomidine Alone in Donkeys Undergoing Field Castration under Total Intravenous Anesthesia IE Helal, HA Al-Abbadi, MA Hashem, HMA Abdelrazek,/,2024

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.

Gene References into Functions

These data suggest that IL-6 may play a key role in equine metabolic syndrome (EMS), and that pro-inflammatory cytokines levels in serum may serve as an additional tool for diagnosing EMS. PMID:25269712
IL-6 stimulation decreased chondrocyte expression of the canonical Wnt signaling pathway transactivator beta-catenin, induced expression of inhibitors of the Wnt pathway, and increased expression of GDF-5. PMID:24471749
study shows that IL-6 is rapidly induced in BAL-cells of airway-compromised horses in response to adenosine exposure, probably through A2BAR activation and that this effect can be modulated by A2AAR PMID:21496101
Our data show the presence of a polymorphism downstream of the equine IL-6 gene that is associated with the basal Cu:Zn ratio in horses independent of breed. PMID:21330167
Expression levels of IL-6 are significantly increased in peripheral blood mononuclear cells from trained horses compared to sedentary animals. PMID:21255427
Failure of passive transfer may directly influence the serum IL-6 concentration in septic foals. Neither serum IL-6 nor IL-10 alone, were useful diagnostic indices of sepsis in equine neonates. PMID:19501415
The contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of IL-6, IL-10 and TNF-alpha, in horses with recurrent airway obstruction during exacerbation and in remission is reported. PMID:18294392
The effects of semen extender and seminal plasma on the expression of inflammatory modulators in the endometrium of mares are reported. PMID:18584861