Hamster interferon, gamma (IFNG) ELISA kit

Product Details

Customer Reviews and Q&A

Citations

Target Background

Name: Hamster interferon, gamma (IFNG) ELISA kit
Brand: CUSABIO
SKU: CSB-EL011050HA

Promotion

Code: CSB-EL011050HA

Rate （N/A）
Review （0）
Citation （3）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenate

Detect Range: 1.87 pg/mL-120 pg/mL

Sensitivity: 0.46 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Alternative Names

IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit

Abbreviation

IFNG

Uniprot NO.

O35497

Species

Mesocricetus auratus (Golden hamster)

Sample Type

serum, plasma, tissue homogenate

Detection Range

1.87 pg/mL-120 pg/mL

Sensitivity

0.46 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of hamster IFNG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	84
	Range %	80-88
1:4	Average %	89
	Range %	84-93
1:8	Average %	98
	Range %	94-102

Recovery

The recovery of hamster IFNG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	92	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
120	2.584	2.727	2.656	2.512
60	2.028	2.106	2.067	1.923
30	1.265	1.334	1.300	1.156
15	0.714	0.755	0.735	0.591
7.5	0.371	0.347	0.359	0.215
3.75	0.293	0.309	0.301	0.157
1.87	0.207	0.217	0.212	0.068
0	0.146	0.142	0.144

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

Interferon gamma (IFN-gamma) is a key cytokine produced primarily by activated T helper 1 cells, cytotoxic T lymphocytes, and natural killer cells. This pleiotropic molecule plays important roles in immune regulation, including macrophage activation, enhancement of antigen presentation, promotion of Th1 cell differentiation, and coordination of cellular immune responses against intracellular pathogens and tumors. IFN-gamma also shows antiviral properties and influences various physiological processes including cell growth regulation and apoptosis, making it a valuable biomarker in immunological and inflammatory research.

The Hamster interferon, gamma (IFNG) ELISA kit (CSB-EL011050HA) provides quantitative measurement of IFN-gamma in Mesocricetus auratus samples using a sandwich assay principle. This kit works with serum, plasma, and tissue homogenate samples with a detection range of 1.87 pg/mL to 120 pg/mL and sensitivity of 0.46 pg/mL. The assay requires 50-100 μL sample volume, completes within 1-5 hours, and uses detection at 450 nm wavelength for reliable quantification of hamster IFN-gamma levels.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in immunotherapy and cancer research studies using Syrian hamster models to quantify interferon gamma production. The kit allows measurement of this key cytokine in cell culture supernatants and tissue homogenates to determine immune responses and therapeutic efficacy.

• Cancer immunotherapy research: Measuring interferon gamma levels in splenocyte cultures following various treatment combinations including checkpoint inhibitor therapy and viral vector-based interventions
• Immune cell activation studies: Determining cytokine production by lymphocytes cocultured with tumor cells to measure T-cell responses and characterize Th1/Th2 immune profiles
• Respiratory disease research: Quantifying interferon gamma in lung tissue homogenates alongside other inflammatory mediators to profile cytokine expression patterns
• Therapeutic intervention monitoring: Measuring interferon gamma as a biomarker to determine the immunomodulatory effects of experimental treatments in hamster disease models

■ Customer Reviews

Submit a Review here

Relaxin-expressing oncolytic adenovirus induces remodeling of physical and immunological aspects of cold tumor to potentiate PD-1 blockade BK Jung,/,2020
Antiviral Nanobiologic Therapy Remodulates Innate Immune Responses to Highly Pathogenic Coronavirus X Liu,/,2023
Enhanced potency of immune checkpoint inhibitors against poorly immunological solid tumors by immune stimulatory oncolytic adenoviruses-mediated remodeling of the tumor microenvironment HM Ahn, BK Jung, JW Hong, D Hong, A Yoon,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.

Subcellular Location

Secreted.

Protein Families

Type II (or gamma) interferon family

Tissue Specificity

Released primarily from activated T lymphocytes.

Promotions