Hamster C-reactive protein(CRP) ELISA Kit

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Name: Hamster C-reactive protein(CRP) ELISA Kit
Brand: CUSABIO
SKU: CSB-EL005991HA

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Code: CSB-EL005991HA

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 12.5 ng/mL-800 ng/mL

Sensitivity: 3.12 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

CRP ELISA kit; PTX1C-reactive protein ELISA kit

Abbreviation

CRP

Uniprot NO.

P49262

Species

Mesocricetus auratus (Golden hamster)

Sample Type

serum, plasma, tissue homogenates

Detection Range

12.5 ng/mL-800 ng/mL

Sensitivity

3.12 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of hamster CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	85-96
1:10	Average %	87
	Range %	82-92
1:20	Average %	96
	Range %	89-103
1:40	Average %	98
	Range %	94-104

Recovery

The recovery of hamster CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-93
EDTA plasma (n=4)	96	87-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	2.017	2.075	2.046	1.888
400	1.592	1.584	1.588	1.430
200	1.062	1.036	1.049	0.891
100	0.625	0.586	0.606	0.448
50	0.456	0.441	0.449	0.291
25	0.309	0.293	0.301	0.143
12.5	0.231	0.218	0.225	0.067
0	0.155	0.161	0.158

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Hamster CRP ELISA Kit was designed for the quantitative measurement of Hamster CRP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Subcellular Location

Secreted.

Protein Families

Pentraxin family

Tissue Specificity

Found in plasma.

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