Guinea pig insulin (INS) ELISA kit

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Name: Guinea pig insulin (INS) ELISA kit
Brand: CUSABIO
SKU: CSB-EL011742GU

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Code: CSB-EL011742GU

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 12 μIU/mL-210 μIU/mL

Sensitivity: 7.5 μIU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit

Abbreviation

INS

Uniprot NO.

P01329

Species

Cavia porcellus (Guinea pig)

Sample Type

serum, plasma, tissue homogenates

Detection Range

12 μIU/mL-210 μIU/mL

Sensitivity

7.5 μIU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-97
1:2	Average %	96
	Range %	90-106
1:4	Average %	95
	Range %	86-104
1:8	Average %	90
	Range %	87-94

Recovery

The recovery of guinea pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-101
EDTA plasma (n=4)	95	89-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
12	0.049	0.051	0.050
24	0.165	0.160	0.163
48	0.377	0.374	0.376
120	0.853	0.849	0.851
210	1.686	1.691	1.689

Troubleshooting and FAQs

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ELISA Data Analysis

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Guinea pig insulin (INS) ELISA kit

Description

This Guinea pig INS ELISA Kit was designed for the quantitative measurement of Guinea pig INS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12 μIU/mL-210 μIU/mL and the sensitivity is 7.5 μIU/mL.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Subcellular Location

Secreted.

Protein Families

Insulin family

Database Links

STRING: 10141.ENSCPOP00000019016

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