Goat Thyroid Stimulating Hormone,TSH ELISA Kit

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Name: Goat Thyroid Stimulating Hormone,TSH ELISA Kit
Brand: CUSABIO
SKU: CSB-E13278G

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Code: CSB-E13278G

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.5 μIU/mL-20 μIU/mL

Sensitivity: 0.25 μIU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

CGAGlycoprotein hormones alpha chain ELISA Kit; Anterior pituitary glycoprotein hormones common subunit alpha ELISA Kit; Follicle-stimulating hormone alpha chain ELISA Kit; FSH-alpha ELISA Kit; Follitropin alpha chain ELISA Kit; Luteinizing hormone alpha chain ELISA Kit; LSH-alpha ELISA Kit; Lutropin alpha chain ELISA Kit; Thyroid-stimulating hormone alpha chain ELISA Kit; TSH-alpha ELISA Kit; Thyrotropin alpha chain ELISA Kit

Abbreviation

CGA

Uniprot NO.

Q8WMW8

Species

Capra hircus (Goat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.5 μIU/mL-20 μIU/mL

Sensitivity

0.25 μIU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	94
	Range %	91-98
1:4	Average %	102
	Range %	97-105
1:8	Average %	92
	Range %	84-98

Recovery

The recovery of goat TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-97
EDTA plasma (n=4)	105	102-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
20	2.122	2.278	2.200
10	1.238	1.204	1.221
5	0.652	0.663	0.658
2.5	0.368	0.347	0.358
1	0.153	0.158	0.156
0.5	0.083	0.081	0.082

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Goat CGA ELISA Kit was designed for the quantitative measurement of Goat CGA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.5 μIU/mL-20 μIU/mL and the sensitivity is 0.25 μIU/mL.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH and follitropin/follicle stimulating hormone/FSH. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.

Subcellular Location

Secreted.

Protein Families

Glycoprotein hormones subunit alpha family

Database Links

UNIGENE: Chi.38024

KEGG: chx:100860817

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