Fish Interleukin 6,IL-6 ELISA Kit

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Name: Fish Interleukin 6,IL-6 ELISA Kit
Brand: CUSABIO
SKU: CSB-E13258Fh

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Code: CSB-E13258Fh

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 12.5 pg/mL-200 pg/mL

Sensitivity: 12.5 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Name

Interleukin 6,IL-6

Alternative Names

il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit

Abbreviation

IL-6

Uniprot NO.

A0S0B0

Species

Fish

Sample Type

serum, plasma, tissue homogenates

Detection Range

12.5 pg/mL-200 pg/mL

Sensitivity

12.5 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	89
	Range %	82-94
1:4	Average %	103
	Range %	94-107
1:8	Average %	96
	Range %	90-101

Recovery

The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	91-104
EDTA plasma (n=4)	91	84-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.128	0.133	0.131
100	0.269	0.258	0.264
50	0.449	0.431	0.440
25	0.851	0.842	0.847
12.5	1.813	1.783	1.798

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

This Fish IL-6 ELISA Kit was designed for the quantitative measurement of Fish IL-6 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 12.5 pg/mL-200 pg/mL and the sensitivity is 12.5 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.

Subcellular Location

Secreted.

Protein Families

IL-6 superfamily

Tissue Specificity

Expressed in kidney and spleen. Low expression in liver and gills.

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