Fish Acetylcholinesterase(AChE)ELISA Kit

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Code: CSB-E17001Fh

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 2.5 ng/mL-40 ng/mL

Sensitivity: 2.5 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details
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Target Background

Product Details

Alternative Names

acheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit

Abbreviation

AChE

Uniprot NO.

P04058

Species

Fish

Sample Type

serum, plasma, tissue homogenates

Detection Range

2.5 ng/mL-40 ng/mL

Sensitivity

2.5 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-103
1:2	Average %	92
	Range %	87-101
1:4	Average %	93
	Range %	87-100
1:8	Average %	89
	Range %	83-99

Recovery

The recovery of fish AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	87-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
40	0.284	0.292	0.288
20	0.521	0.532	0.527
10	0.886	0.891	0.889
5	1.374	1.382	1.378
2.5	2.583	2.599	2.591

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Fish AChE ELISA Kit was designed for the quantitative measurement of Fish AChE protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 ng/mL-40 ng/mL and the sensitivity is 2.5 ng/mL.

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Citations

Target Background

Function(From Uniprot)

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.

Subcellular Location

[Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.; [Isoform T]: Cell membrane; Peripheral membrane protein. Cell junction, synapse. Note=Attached to the membrane through disulfide linkage with the collagenic subunit, itself bound to the membrane.

Protein Families

Type-B carboxylesterase/lipase family

Tissue Specificity

Found in the synapses and to a lower extent in extrajunctional areas of muscle and nerve, and on erythrocyte membranes.

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