Chicken growth hormone,GH ELISA KIT

Chicken growth hormone,GH ELISA KIT

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Name: Chicken growth hormone,GH ELISA KIT
Brand: CUSABIO
SKU: CSB-E09866Ch

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Code: CSB-E09866Ch

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 625 pg/mL-10000 pg/mL

Sensitivity: 625 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit

Abbreviation

Uniprot NO.

P08998

Species

Gallus gallus (Chicken)

Sample Type

serum, plasma, tissue homogenates

Detection Range

625 pg/mL-10000 pg/mL

Sensitivity

625 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Developmental Biology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	99
	Range %	92-105
1:4	Average %	85
	Range %	80-89
1:8	Average %	90
	Range %	83-98

Recovery

The recovery of chicken GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-96
EDTA plasma (n=4)	94	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
10000	0.154	0.145	0.150
5000	0.261	0.244	0.253
2500	0.538	0.534	0.536
1250	1.113	1.139	1.126
625	2.356	2.278	2.317

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Chicken growth hormone,GH ELISA KIT

Description

This Chicken growth hormone (GH) ELISA Kit was designed for the quantitative measurement of Chicken growth hormone (GH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 625 pg/mL-10000 pg/mL and the sensitivity is 625 pg/mL.

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Function(From Uniprot)

Growth hormone plays an important role in growth control.

Gene References into Functions

This is the first study to demonstrate that single nucleotide polymorphisms in GHSR can increase appetite, growth traits, expression and level of GHRL, suggesting a hunger signal role for endogenous GHRL. PMID:27591070
There is a negative glucocorticoid response-like element located in the +591/+609 region within the first intron of chicken growth hormone (cGH), which is essential for the down-regulation of cGH expression. PMID:27834851
It is shown that in all examined chicken populations the growth hormone gene is polymorphic. PMID:26841491
The molecular mechanisms underlying glucocorticoid recruitment of somatotrophs during embryogenesis were studied; these provide insight into glucocorticoid-induced developmental changes in other tissues and cell types. PMID:25560830
T185G growth hormone polymorphism was significantly associated with the egg production traits in chickens. PMID:25062422
GHRH stimulates testicular GH secretion in an autocrine or paracrine manner. PMID:24508498
The results indicate that high frequency of I, C and A alleles corresponds to the egg-laying chicken lines. PMID:23285753
A 1727-bp fragment of 5'-flanking region of chicken growth hormone (cGH) gene has been cloned and sequenced. PMID:15229358
small chicken GH expression and translation occurs in ocular tissues of chick embryos, in which its localization in the neural retina and the optic nerve head is distinct from that of the full-length protein PMID:17008400
The identification of 3 restriction enzyme polymorphic sites were detected in the growth hormone gene is reported. PMID:17495076
Results showed that there was no significant difference between dwarf and normal chickens in GH expression. PMID:17681929
results demonstrate presence of growth hormone and its receptors in the ovarian stroma with cortical follicles & in the wall of small and large follicles of the ovary during sexual maturation PMID:18335240
Positive somatotroph responses to corticosterone (CORT) are lost during chicken embryonic development and that glucocorticoid receptors are the primary regulator of CORT-induced growth hormone secretion. PMID:19157766

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Subcellular Location

Secreted.

Protein Families

Somatotropin/prolactin family

Database Links

UNIGENE: Gga.48744

KEGG: gga:378781

STRING: 9031.ENSGALP00000000327

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.