Chicken Interleukin 8(IL-8) ELISA Kit

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Citations

Target Background

Name: Chicken Interleukin 8(IL-8) ELISA Kit
Brand: CUSABIO
SKU: CSB-E14191C

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Code: CSB-E14191C

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Citation （8）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 1.56 pg/mL-400 pg/mL

Sensitivity: 0.78 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Target Name

interleukin 8

Alternative Names

CXCL8 ELISA Kit; CEF4 ELISA Kit; EMF1 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; 9E3 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Embryo fibroblast protein 1 ELISA Kit; EMF-1 ELISA Kit

Abbreviation

CXCL8

Uniprot NO.

P08317

Species

Gallus gallus (Chicken)

Sample Type

serum, plasma, tissue homogenates

Detection Range

1.56 pg/mL-400 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	97-106
1:2	Average %	88
	Range %	84-93
1:4	Average %	104
	Range %	99-109
1:8	Average %	103
	Range %	99-106

Recovery

The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	91	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
400	0.187	0.183	0.185
100	0.259	0.267	0.263
25	0.471	0.452	0.462
6.25	0.862	0.843	0.853
1.56	1.410	1.398	1.404
0	2.251	2.159	2.205

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

Interleukin 8 (IL-8), also known as CXCL8, is a pro-inflammatory chemokine that plays a key role in immune responses and inflammation. This cytokine primarily recruits and activates neutrophils at sites of infection or tissue damage. Various cell types produce IL-8, including macrophages, epithelial cells, and endothelial cells, when exposed to inflammatory stimuli. In avian species, IL-8 serves similar functions to its mammalian counterpart. It mediates innate immune responses and contributes to the inflammatory cascade during pathogen encounters or tissue injury.

The Chicken Interleukin 8(IL-8) ELISA Kit (CSB-E14191C) is designed for quantitative measurement of interleukin 8 in Gallus gallus samples. This competitive assay works with serum, plasma, and tissue homogenates. The detection range spans 1.56 pg/mL-400 pg/mL with sensitivity of 0.78 pg/mL. The assay requires 50-100ul sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for IL-8 quantification in chicken samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in avian immunology research to quantify interleukin 8 levels in chicken biological samples. The applications span inflammatory response studies and immune system profiling in poultry research models.

• Inflammatory response studies - Measurement of IL-8 concentrations in serum and plasma samples to assess pro-inflammatory cytokine profiles during infection models and immune challenges
• Infection research - Quantification of IL-8 levels in chickens following bacterial infection to monitor inflammatory biomarker responses over time
• Immune profiling - Multi-cytokine analysis incorporating IL-8 measurements alongside other inflammatory mediators to characterize comprehensive immune responses
• Poultry immunology - Study of cytokine expression patterns in different chicken strains to understand breed-specific immune characteristics and stress responses

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■ Q&A

Can I get a copy of the kit instructions to as well as any references to papers that have used this kit? I want to make sure it will work in our specific use case. To that end I'm looking for information about the nature of the antibodies (capture and detection) are they monoclonal or poly. Are they the same Ab for both steps or different? Are the plates recoated, or do we need to coat them ourselves? Thank you for the help.

Thanks for your inquiry.
I will send you the manual by e-mail.
Here is the reference about this kit: https://www.sciencedirect.com/science/article/pii/S0165242717302362
The plate is ready to use and you don't need to coat it by yourself. The plate is coated with specific antigen.There is only one antibody in the kit and it is goat polyclonal antibody.
Pls let me know if you have any further questions. Thank you.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

May be an autocrine factor that promotes the growth of fibroblasts and is involved in the neoplastic transformation of fibroblasts by v-Src. Chemotactic for peripheral blood mononuclear cells as well as for heterophils.

Gene References into Functions