Chicken Insulin ELISA kit

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Name: Chicken Insulin ELISA kit
Brand: CUSABIO
SKU: CSB-E13293C

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Code: CSB-E13293C

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Citation （7）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 3.12 μIU/mL-50 μIU/mL

Sensitivity: 0.59 μIU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit

Abbreviation

INS

Uniprot NO.

P67970

Species

Gallus gallus (Chicken)

Sample Type

serum, plasma, tissue homogenates

Detection Range

3.12 μIU/mL-50 μIU/mL

Sensitivity

0.59 μIU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken insulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	96
	Range %	90-101
1:4	Average %	98
	Range %	95-103
1:8	Average %	90
	Range %	85-96

Recovery

The recovery of chicken insulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	97	95-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
50	0.141	0.145	0.143
25	0.310	0.313	0.312
12.5	0.685	0.676	0.681
6.25	1.477	1.472	1.475
3.12	2.709	2.707	2.708

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

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Chicken Insulin ELISA kit

Description

Insulin is a peptide hormone produced primarily by pancreatic beta cells that plays a fundamental role in glucose homeostasis and metabolic regulation. This hormone promotes cellular glucose uptake, stimulates glycogen synthesis, and inhibits gluconeogenesis, making it essential for maintaining blood glucose levels within physiological ranges. Insulin signaling pathways are extensively studied in diabetes research, metabolic disorders, and endocrinology. Dysregulation of insulin production or sensitivity leads to various pathological conditions including type 1 and type 2 diabetes mellitus.

The Chicken Insulin ELISA kit (CSB-E13293C) is designed for quantitative measurement of insulin (INS) in Gallus gallus samples. This competitive assay works with serum, plasma, and tissue homogenates with a detection range of 3.12 μIU/mL to 50 μIU/mL and sensitivity of 0.59 μIU/mL. The assay requires 50-100 μL sample volume, operates at 450 nm detection wavelength, and can be completed within 1-5 hours, providing researchers with a reliable tool for insulin quantification in avian studies.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This chicken insulin ELISA kit has been used in avian research studies focused on metabolic and endocrine profiling in laying hens. The kit allows quantification of insulin levels in serum samples as part of biochemical analyses examining hormonal and inflammatory biomarkers. Research applications have included studies examining the relationships between insulin and other metabolic regulators in poultry models.

• Metabolic research: Measurement of insulin concentrations alongside glucose measurements and other metabolic parameters in laying hen studies
• Endocrine profiling: Multi-analyte serum biochemical analyses incorporating insulin measurement with growth factors, hormones, and bone metabolism markers
• Inflammatory biomarker studies: Analysis of insulin levels in conjunction with pro-inflammatory cytokines and other immune-related factors in avian models
• Longitudinal monitoring: Serial insulin measurements across experimental timepoints to track metabolic changes in poultry research

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■ Q&A

Regarding your ELISA kit CSB-E13293C, could you please advise on the following:
1) Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Does the standard include additive agent such as antiseptic or preservative? If it includes, what is the composition of said additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

Thanks for your inquiry.
CSB-E13293C
Standard：293 cell-derived recombinant protein.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
The kit doesn't contain hazardous substance such as azide, thimerosal, 2-ME, etc.
Pls let me know if you have any further questions. Thank you.

Identification of the transcript isoforms and expression characteristics for chicken Lpin1 Wang XK et al,/,2012
Effect of Energy Restriction on Growth, Slaughter Performance, Serum Biochemical Parameters and Lpin2/WDTC1 mRNA Expression of Broilers in the Later Phase Wen Chen et al,/,2012
Effect of short-and long-term feed restriction on ghrelin concentrations in turkeys F R Vizcarra.et al,/,2018
Dietary vegetable oils inclusion on the performance, hormonal levels and hsp 70 gene expression in broilers under heat stress Tari A R, et al,/,2019
Insulin resistance and metabonomics analysis of fatty liver haemorrhagic syndrome in laying hens induced by a high-energy low-protein diet Zhuang Y, et al,/,2019
Alpha-Ketoglutarate: An Effective Feed Supplement in Improving Bone Metabolism and Muscle Quality of Laying Hens: A Preliminary Study E Tomaszewska,/,2020
ß-Hydroxy-ß-methylbutyrate: A feed supplement influencing performance, bone metabolism, intestinal morphology, and muscle quality of laying hens: a preliminary one-point study E Tomaszewska,/,2024

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Gene References into Functions

Data suggest that insulin signaling in skeletal muscle can be regulated by dietary factors (here, dietary content of omega-3 fatty acids). PMID:24094087
Polymorphisms within the INS gene are associated with 55-week body weight. PMID:16734694
The present findings suggest the involvement of insulin in neuronal/glial coupling in the cerebellum. PMID:19135128

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Subcellular Location

Secreted.

Protein Families

Insulin family

Database Links

UNIGENE: Gga.673

KEGG: gga:396145

STRING: 9031.ENSGALP00000010567

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