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Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor,(MCP-1/CCL2/MCAF)ELISA Kit

Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor,(MCP-1/CCL2/MCAF)ELISA Kit

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Code: CSB-E16221B

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 78 pg/mL-5000 pg/mL

Sensitivity: 0.039 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Product Details

Target Name

chemokine (C-C motif) ligand 2

Alternative Names

CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Acidic seminal fluid protein ELISA Kit; Monocyte chemotactic protein 1A ELISA Kit; MCP-1 ELISA Kit; MCP-1A ELISA Kit; Small-inducible cytokine A2 ELISA Kit

Abbreviation

CCL2

Uniprot NO.

P28291

Species

Bos taurus (Bovine)

Sample Type

serum, plasma, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

Recovery

Typical Data

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Bovine CCL2 ELISA Kit is a tool for the quantification of C-C motif chemokine 2 (CCL2) in bovine serum, plasma, and tissue homogenates. CCL2 is a protein that plays a critical role in immune responses, particularly in the recruitment of immune cells to sites of inflammation. This ELISA kit detects CCL2 with high sensitivity, with a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5pg/ml .

The assay principle is a sandwich measurement, providing quantitative results that can be obtained in as little as 1-5 hours. The kit is optimized for use with a sample volume of 50-100ul, making it a cost-effective and efficient solution for researchers.

CCL2 has been shown to be a reliable biomarker of immune responses. and this kit provides researchers with a precise and reliable method to accurately measure CCL2 levels in bovine samples.

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Target Background

Function(From Uniprot)

Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.

Gene References into Functions

CCL2 plays a pivotal role in reproductive tissues and may support maternal-fetal interface to improve efficiency of pregnancy. PMID:28816360
resence of type 2 diabetes negatively affects the work of cytokines and markers of fibrosis, as evidenced by higher levels of interleukin-1beta and monocyte chemoattractant protein-1, compared with patients without diabetes PMID:25020170
Studied SNPs in the bovine toll-like receptor 4 (TLR4) and monocyte chemo attractant protein-1(CCL2) genes. PMID:24813219
The LEP, IGF2 and CCL2 genes showed allelic expression imbalance in liver, kidney and pituitary glands of Polish Holstein-Friesian bulls. PMID:23184004
immune cells and endothelial cells can interact cooperatively to increase CCL2 secretion in the corpus luteum PMID:15659710