Bovine lipopolysaccharide binding protein (LBP) ELISA kit

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Bovine lipopolysaccharide binding protein (LBP) ELISA kit

Name: Bovine lipopolysaccharide binding protein (LBP) ELISA kit
Brand: CUSABIO
SKU: CSB-E16370B

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Code: CSB-E16370B

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 15.6 ng/mL-1000 ng/mL

Sensitivity: 3.9 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

LBPLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit

Abbreviation

LBP

Uniprot NO.

Q2TBI0

Species

Bos taurus (Bovine)

Sample Type

serum, plasma, tissue homogenates

Detection Range

15.6 ng/mL-1000 ng/mL

Sensitivity

3.9 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-102
1:2	Average %	86
	Range %	82-95
1:4	Average %	98
	Range %	90-107
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of bovine LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	95	90-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.126	2.150	2.138	2.002
500	1.611	1.623	1.617	1.481
250	1.142	1.158	1.150	1.014
125	0.797	0.780	0.789	0.653
62.5	0.463	0.451	0.457	0.321
31.25	0.340	0.334	0.337	0.201
15.6	0.271	0.275	0.273	0.137
0	0.135	0.136	0.136

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Bovine lipopolysaccharide binding protein (LBP) ELISA kit

Description

This Bovine LBP ELISA Kit was designed for the quantitative measurement of Bovine LBP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

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■ Q&A

I am interested in the product CSB-E16370B and would like to confirm:
Has this kit actually been validated with bovine samples, or is it based off a sequence homology?

Thanks for your inquiry.
We tested bovine serum and plasma samples before and the test value is 15.6-62.5ng/ml.
We suggest you use the kit for the corresponding species to test your samples.
Pls let me know if you have any further questions. Thank you.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.

Gene References into Functions

Our study provides an overview of the gene expression profile between wild LBP and mutant LBP on the LPS-induced inflammatory response of bovine mammary epithelial cells , which will lead to further understanding of the potential effects of LBP mutations on bovine mammary glands. PMID:26813383
The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID:25542063
One possible anti-inflammatory mechanism can be attributed to the negative role of BRLBP in suppressing TLR4/NF-kappaB activation mediated by LPS. These findings suggested that BRLBP may be a useful agent to treat LPS-induced mastitis PMID:25700343
Single nucleotide polymorphisms in the lipopolysaccharide-binding protein may hold the secret of susceptibility to clinical mastitis in Chinese Holstein. PMID:22733492