Bovine Transthyretin(TTR) ELISA kit

Product Details

Name: Bovine Transthyretin(TTR) ELISA kit
Brand: CUSABIO
SKU: CSB-EL025270BO

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Code: CSB-EL025270BO

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 312.5 ng/mL-5000 ng/mL

Sensitivity: 312.5 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Alternative Names

TTR ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit

Abbreviation

TTR

Uniprot NO.

O46375

Species

Bos taurus (Bovine)

Sample Type

serum, plasma, tissue homogenates

Detection Range

312.5 ng/mL-5000 ng/mL

Sensitivity

312.5 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-97
1:200	Average %	103
	Range %	99-107
1:400	Average %	98
	Range %	93-103
1:800	Average %	92
	Range %	87-95

Recovery

The recovery of bovine TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	89	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
5000	0.147	0.153	0.150
2500	0.266	0.275	0.271
1250	0.511	0.512	0.512
625	0.905	0.903	0.904
312.5	1.674	1.787	1.731
0	2.689	2.599	2.644

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ELISA Data Analysis

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Bovine Transthyretin(TTR) ELISA kit

Description

This Bovine TTR ELISA Kit was designed for the quantitative measurement of Bovine TTR protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 312.5 ng/mL-5000 ng/mL and the sensitivity is 312.5 ng/mL.