Bovine Interleukin 2,IL-2 ELISA Kit

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Name: Bovine Interleukin 2,IL-2 ELISA Kit
Brand: CUSABIO
SKU: CSB-E09810b

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Code: CSB-E09810b

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Citation （8）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 31.25 pg/mL-2000 pg/mL

Sensitivity: 7.8 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit

Abbreviation

IL2

Uniprot NO.

P05016

Species

Bos taurus (Bovine)

Sample Type

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-90
1:2	Average %	100
	Range %	97-103
1:4	Average %	101
	Range %	98-105
1:8	Average %	94
	Range %	86-98

Recovery

The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	92-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.151	2.099	2.125	2.040
1000	1.683	1.656	1.670	1.585
500	1.284	1.270	1.277	1.192
250	0.846	0.833	0.840	0.755
125	0.502	0.498	0.500	0.415
62.5	0.367	0.356	0.362	0.277
31.25	0.221	0.213	0.217	0.132
0	0.086	0.084	0.085

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

Interleukin 2 (IL-2) is a cytokine that plays a central role in immune system regulation and T cell activation. Originally discovered as a T cell growth factor, IL-2 is primarily produced by activated CD4+ T helper cells and serves as a key mediator of adaptive immune responses. This cytokine promotes T cell proliferation, differentiation, and survival, while also influencing the development and function of regulatory T cells. IL-2 signaling occurs through the IL-2 receptor complex and activates multiple downstream pathways that maintain immune homeostasis and mount effective immune responses against pathogens.

The Bovine Interleukin 2, IL-2 ELISA Kit (CSB-E09810b) is designed for quantitative measurement of IL-2 in Bos taurus samples. This sandwich-based assay accommodates serum, plasma, and tissue homogenates with a detection range of 31.25 pg/mL to 2000 pg/mL and sensitivity of 7.8 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength. This provides researchers with a reliable tool for studying bovine IL-2 levels in various biological samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This bovine interleukin 2 ELISA kit has been used in research focused on immune function and inflammatory response monitoring in cattle. The kit allows quantitative measurement of interleukin 2 concentrations in bovine biological samples as part of comprehensive cytokine profiling studies.

• Immune function assessment - Measurement of interleukin 2 alongside other cytokines and immunoglobulins to evaluate overall immune status in bovine subjects
• Inflammatory biomarker profiling - Quantification of interleukin 2 as part of multi-analyte panels including various interleukins and tumor necrosis factor to assess inflammatory responses
• Serum-based cytokine analysis - Detection of interleukin 2 concentrations in bovine serum samples for immunological research applications

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Dietary effects of lutein-fortified chlorella on milk components of Holstein cows. Jeon JY.et al,/,2016
Kinetics of selected plasma cytokines during innate-adaptive immune response transition in adult cattle infected with the bovine ephemeral fever virus R. Barigye.et al,/,2016
Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows Seid Mohammed.et al,/,2017
Enhancing dietary cation-anion difference reshaped the distribution of endotoxin across different biofluids and influenced inflammatory response in dairy cows … Z.Wang,/,2020
Complete genome sequence and antimicrobial activity of Bacillus velezensis JT3-1, a microbial germicide isolated from yak feces Y Li,/,2020
Association of the MACROD2 rs6110695 A>G polymorphism with an increasing WBC count in a Korean population J Yang,/,2022
The relationship between serum cytokine profile and vitamin D in calves with neonatal diarrhea M Caliskan,/,2023
Immunological response enhancement in cows with subclinical mastitis fed diet supplemented with Macleaya cordata MSA Khattab, L Haiqiang, T Shaoxun,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Gene References into Functions

Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor. PMID:12906107

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Subcellular Location

Secreted.

Protein Families

IL-2 family

Database Links

UNIGENE: Bt.341

KEGG: bta:280822

STRING: 9913.ENSBTAP00000027819

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