Bovine Interleukin 12,IL-12 ELISA Kit

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Name: Bovine Interleukin 12,IL-12 ELISA Kit
Brand: CUSABIO
SKU: CSB-E14010B

CSB-E14010B

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 6.25 pg/mL-400 pg/mL

Sensitivity: 1.56 pg/mL

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Orders can only be placed from within the U.S.

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Alternative Names

IL12AInterleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit

Abbreviation

IL12A

Uniprot NO.

P54349

Species

Bos taurus (Bovine)

Sample Type

serum, plasma, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	98
	Range %	91-102
1:4	Average %	94
	Range %	90-98
1:8	Average %	94
	Range %	86-99

Recovery

The recovery of bovine IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-92
EDTA plasma (n=4)	97	95-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.758	2.628	2.693	2.531
200	2.184	2.084	2.134	1.972
100	1.612	1.562	1.587	1.425
50	1.203	1.173	1.188	1.026
25	0.652	0.642	0.647	0.485
12.5	0.384	0.374	0.379	0.217
6.25	0.258	0.253	0.256	0.094
0	0.163	0.161	0.162

Troubleshooting and FAQs

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Datasheet & COA

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Description

This Bovine IL12A ELISA Kit was designed for the quantitative measurement of Bovine IL12A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Gene References into Functions

The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID:17530193
Lipopolysaccharide stimulation up-regulates the secretion of cytokines, including interleukin-12, by bovine polymorphonuclear neutrophil leukocytes. PMID:17727806

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Subcellular Location

Secreted.

Protein Families

IL-6 superfamily

Database Links

UNIGENE: Bt.524

KEGG: bta:281856

STRING: 9913.ENSBTAP00000020153

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