Bovine Deoxyribonuclease-1(DNASE1) ELISA kit

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Code: CSB-EL007049BO

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 12.5 pg/mL-800 pg/mL

Sensitivity: 3.1 pg/mL

Trial Size:

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Target Background

Product Details

Alternative Names

DNASE1 ELISA Kit; DNL1Deoxyribonuclease-1 ELISA Kit; EC 3.1.21.1 ELISA Kit; Deoxyribonuclease I ELISA Kit; DNase I ELISA Kit

Abbreviation

DNASE1

Uniprot NO.

P00639

Species

Bos taurus (Bovine)

Sample Type

serum, plasma, tissue homogenates

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.1 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine DNASE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	85
	Range %	80-89
1:40	Average %	94
	Range %	90-98
1:80	Average %	103
	Range %	99-109
1:160	Average %	97
	Range %	92-101

Recovery

The recovery of bovine DNASE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	93	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.399	2.416	2.408	2.273
400	2.213	2.195	2.204	2.069
200	1.813	1.741	1.777	1.642
100	1.204	1.227	1.216	1.081
50	0.683	0.713	0.698	0.563
25	0.400	0.392	0.396	0.261
12.5	0.292	0.284	0.288	0.153
0	0.131	0.138	0.135

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

This Bovine DNASE1 ELISA Kit was designed for the quantitative measurement of Bovine DNASE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.1 pg/mL.

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Target Background

Function(From Uniprot)

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.

Gene References into Functions

ascorbic acid has the ability to inhibit DNase I, one of the main endonucleases involved in DNA fragmentation during apoptosis. PMID:29551685
the practical capability of this assay system was successfully demonstrated by its use to determine DNase I activity in bovine urine. PMID:27570054
Specificity in DNA-DNase I interaction is mediated by DNA flexibility, which influences the induced-fit transitions required to form productive complexes. PMID:19850052
results indicated that the backbone hydrogen bonding in the antiparallel beta-sheet formed by its N- and C-termini, rather than the side-chain interactions, is crucial for the correct protein folding PMID:15311926