Bovine Caspase 7(Casp-7) ELISA Kit

Product Details

Name: Bovine Caspase 7(Casp-7) ELISA Kit
Brand: CUSABIO
SKU: CSB-E17315B

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Code: CSB-E17315B

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.625 ng/mL-40 ng/mL

Sensitivity: 0.156 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Target Name

Caspase 7(Casp-7)

Alternative Names

Abbreviation

Casp7

Uniprot NO.

F1MD58

Species

Bos taurus (Bovine)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine Casp-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-101
1:2	Average %	95
	Range %	88-102
1:4	Average %	89
	Range %	83-100
1:8	Average %	93
	Range %	89-106

Recovery

The recovery of bovine Casp-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-112
EDTA plasma (n=4)	93	84-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.546	2.457	2.502	2.369
20	1.883	1.905	1.894	1.761
10	1.136	1.124	1.130	0.997
5	0.726	0.711	0.719	0.586
2.5	0.407	0.397	0.402	0.269
1.25	0.305	0.313	0.309	0.176
0.625	0.196	0.204	0.200	0.067
0	0.130	0.136	0.133

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Bovine Casp7 ELISA Kit was designed for the quantitative measurement of Bovine Casp7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.