fbpA Antibody

CSB-PA182794ZA01GKB

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Mycobacterium tuberculosis

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P9WQP3

Target Names

fbpA

Alternative Names

Diacylglycerol acyltransferase/mycolyltransferase Ag85A (DGAT) (EC 2.3.1.122) (EC 2.3.1.20) (Acyl-CoA:diacylglycerol acyltransferase) (Antigen 85 complex A) (85A) (Ag85A) (Fibronectin-binding protein A) (Fbps A), fbpA, mpt44

Raised in

Rabbit

Species Reactivity

Mycobacterium tuberculosis

Immunogen

Recombinant Mycobacterium tuberculosis fbpA protein

Conjugate

Non-conjugate

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (12-14 weeks)

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Protocols

ELISA Protocol

Datasheet & COA

Antibody FAQs

Description

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Target Background

Function

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.

Gene References into Functions

Ag85A protein can induce strong specific humoral and Th1 type cellular immune responses. PMID:29727142
A screen for protein-protein interactions reveals a functional link between the virulence-associated lipid transporter LprG and the mycolyltransferase antigen 85A. PMID:28276676
Using a prime and pull approach, lentivector vaccines expressing Ag85A induce immunogenicity but fail to induce protection against Mycobacterium bovis bacillus Calmette-Guerin challenge in mice. PMID:26095282
The authors show that the enzyme possesses acyl-CoA:diacylglycerol acyltransferase (DGAT) activity in addition to its well-known function as mycolyltransferase. PMID:21819455
findings revealed 6 proteins with high-affinity for the fbpA promoter, and 7 with specificity the fbpC promoter PMID:19959397
analysis of enzymatic hydrolysis of trehalose dimycolate releases free mycolic acids during mycobacterial growth in biofilms PMID:20375425
In addition to immune recognition of Ag85A whole protein, peptide-mapping studies identified four immunogenic Ag85A peptides, which induced Th-1 immune responses in individual sarcoidosis subjects, suggesting a role in sarcoidosis immunopathogenesis. PMID:17357846