US12 Antibody

Code: CSB-PA360979ZA01HWY

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Size:

20μl50μl100μl

US$83

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Species Reactivity: Human herpesvirus 1

Application: ELISA, WB

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Product Details
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Target Background

Product Details

Uniprot NO.

P03170

Target Names

US12

Alternative Names

ICP47 protein (Immediate-early protein IE12) (Immediate-early-5) (Infected cell protein 47) (US12 protein) (Vmw12), US12

Species Reactivity

Human herpesvirus 1

Immunogen

Recombinant Human herpesvirus 1 ICP47 protein (US12) (1-88aa)

Immunogen Species

Human herpesvirus 1

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in Recombinant protein
All lanes: US12 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13.3 kDa
Observed band size: 15 kDa

Description

In the production of the US12 antibody, a rabbit is immunized with recombinant human herpesvirus 1 ICP47 protein (1-88aa), resulting in the production of IgG antibodies by the rabbit's B lymphocytes. The polyclonal US12 antibody is then subjected to meticulous purification through protein G. This US12 antibody can react with human herpesvirus 1 ICP47 protein in ELISA and WB experiments.

The human herpesvirus 1 (HSV-1) US12-encoding protein ICP47 is a polymorphous protein. HSV-1 ICP47 could interfere with RNA splicing in early infection and then shuttle viral mRNA from the nucleus to the cytoplasm in late infection. HSV-1 ICP47's direct interaction with the antigen-dependent transporter (TAP) restricts antigen trafficking, resulting in the generation of empty MHC-I molecules, thus promoting the viral evasion of the immune system.

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Target Background

Function(From Uniprot)

Plays a role in the inhibition of host immune response. Binds specifically to transporters associated with antigen processing (TAP), thereby blocking peptide-binding and translocation by TAP as well as subsequent loading of peptides onto MHC class I molecules. Empty MHC I molecules are retained in the endoplasmic reticulum and ultimately directed to proteasomal degradation. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.

Gene References into Functions

The structure shows that herpes simplex virus ICP47 traps human TAP in an inactive conformation distinct from the normal transport cycle. PMID:27935481
cryo-electron microscopy structure of human TAP in complex with its inhibitor ICP47, a small protein produced by the herpes simplex virus I PMID:26789246
Results indicate that recombinant adenovirus r-H-ICP47 had the abilities of reducing the proliferation of lymphocytes activated by dendritic cells (DCs). PMID:23774531

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Subcellular Location

Host cytoplasm. Host nucleus.

Protein Families

Herpesviridae US12 family

Database Links

KEGG: vg:2703441

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