Unc13a Antibody

Code: CSB-PA723474XA01RA

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0.1ml

0.1ml1ml

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Species Reactivity: Rattus norvegicus (Rat)

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

Q62768

Target Names

Unc13a

Alternative Names

Unc13a antibody; Unc13h1 antibody; Protein unc-13 homolog A antibody; Munc13-1 antibody

Raised in

Rabbit

Species Reactivity

Rattus norvegicus (Rat)

Immunogen

Recombinant Rattus norvegicus (Rat) Unc13a protein

Immunogen Species

Rattus norvegicus (Rat)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Facilitates neuronal dense core vesicles fusion as well as controls the location and efficiency of their synaptic release. Also involved in secretory granule priming in insulin secretion. Plays a role in dendrite formation by melanocytes.

Gene References into Functions

These results suggested that Munc13-1 has an inhibitory role in antigen-induced mast cell degranulation, which is performed in a Munc13-4-dependent manner. PMID:29225210
W22A, W22K, W22D, W22Y, and W22F substitutions were made in Munc13-1. The GFP-tagged constructs were expressed in Neuro-2a cells. Their membrane translocation in response to phorbol ester was observed in live cells by confocal microscopy. Munc13-1 translocated to the plasma membrane, the C1 domain translocated to internal membranes in response to phorbol ester. Trp-588 is important for ligand binding and translocation. PMID:29244485
The structure imposes key constraints for models of neurotransmitter release and suggests that Munc13-1 bridges the vesicle and plasma membranes from the periphery of the membrane-membrane interface. PMID:28177287
Overall, these results support a model whereby the multiple domains of Munc13 cooperate to coordinate synaptic vesicle docking, priming and fusion. PMID:27213521
Here, the authors identified two conserved residues (R151, I155) in the syntaxin-1 linker region as key sites for the Munc13-1 MUN domain interaction. This interaction is essential for SNARE complex formation in vitro and synaptic vesicle priming in neuronal cultures. PMID:28137749
The crystal structure of the rat Munc13-1 MUN domain now reveals an elongated, arch-shaped architecture formed by alpha-helical bundles, with a highly conserved hydrophobic pocket in the middle. PMID:26030875
The C-terminal module of Munc13-1 is important for Munc13 function, but full activity requires adjacent modules. PMID:22000513
The Munc13-1 MUN domain markedly accelerates the transition from the syntaxin-1-Munc18-1 complex to the SNARE complex. PMID:21499244
metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals PMID:20375012
Data suggest that, during repeated action potentials, Ca(2+) and phosphatidylinositolphosphate binding to the Munc13 C(2)B domain potentiate synaptic vesicle exocytosis, thereby offsetting synaptic depression induced by vesicle depletion. PMID:20154707
Presynaptic silencing was accompanied by decreases in levels of the priming proteins Munc13-1 and Rim1. PMID:20130189
Munc13-1 regulates insulin exocytosis PMID:12871971
A conserved tryptophan residue occludes the diacylglycerol/phorbol ester-binding site in the munc13-1 C1 domain, and as a consequence, the munc13-1 C1 domain requires a considerable conformational change for ligand binding. PMID:15667202
Study shows that the Munc13-1 C(2)A domain homodimerizes, and that homodimerization competes with Munc13-1/RIM heterodimerization. PMID:16732694
Expression, purification and characterization of critical domains of Munc13-1 are reported. PMID:17687497
Munc13s may provide a template to assemble syntaxin-1/SNAP-25 heterodimers, leading to an acceptor complex for synaptobrevin PMID:18201107
These results suggest that Munc13-1 exists in pancreas islets during fetus development and its deficiency in the pancreas, as occurs in IUGR, was in accordance with decreased blood insulin level. PMID:18787382

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic cell membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic active zone. Note=Translocated to the plasma membrane in response to phorbol ester binding.

Protein Families

Unc-13 family

Tissue Specificity

Expressed in brain, with highest levels in the olfactory bulb, striatum, cerebral cortex, hippocampus and cerebellum. Also expressed in pancreatic islet cells.

Database Links

UNIGENE: Rn.10126

KEGG: rno:64829

STRING: 10116.ENSRNOP00000025162

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