SPP1 Antibody

Code: CSB-PA178153XA01SVG

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

Q03012

Target Names

SPP1

Alternative Names

SPP1 antibody; CPS40 antibody; SAF41 antibody; YPL138C antibody; COMPASS component SPP1 antibody; Complex proteins associated with SET1 protein SPP1 antibody; Set1C component SPP1 antibody; Suppressor of PRP protein 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SPP1 protein

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

The COMPASS (Set1C) complex specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3, which subsequently plays a role in telomere length maintenance and transcription elongation regulation.

Gene References into Functions

A model where Spp1 works in three ways to promote recombination initiation: first by depositing histone H3K4 methylation (Set1 complex), next by "reading" and protecting histone H3K4 methylation, and finally by making the link with the chromosome axis (Mer2-Spp1 complex). This work deciphers the precise roles of Spp1 in meiotic recombination. PMID:29444071
Spp1 regulates the catalytic activity of the enzyme (Set1) and mediates its biological effect (meiotic DSB formation) independently of the single Set1 Complex. PMID:23511748
Spp1, a conserved member of the histone H3K4 methyltransferase Set1 complex, is required for normal levels of double strand breaks formation and is associated with chromosome axes during meiosis. PMID:23246437
findings show tethering of Spp1 (a component of the COMPASS complex), to recombinationally cold regions is sufficient to induce double-strand breaks (DSB); by interacting with H3K4me3 and Mer2, Spp1 promotes recruitment of potential meiotic DSB sites to the chromosomal axis PMID:23160953
the origin recognition complex (ORC) mediates histone 3 lysine 4 methylation through cooperation with Spp1 in Saccharomyces cerevisiae PMID:18845545
Data show that Tyr1052, a putative Phe/Tyr switch of Set1, plays an essential role in the regulation of H3K4 trimethylation by COMPASS and that the mutation to phenylalanine (Y1052F) suppresses the loss of Cps40 in H3K4 trimethylation levels. PMID:19398585