SP1 Antibody

Code: CSB-PA973357XA01DOA

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Arabidopsis thaliana

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

Q8L7N4

Target Names

SP1

Alternative Names

SP1 antibody; DIAL1 antibody; At1g63900/At1g63890 antibody; T12P18.8/T12P18.9 antibody; E3 ubiquitin-protein ligase SP1 antibody; EC 2.3.2.27 antibody; DIAP1-like protein 1 antibody; Protein SUPPRESSOR OF PPI1 LOCUS 1 antibody; RING-type E3 ubiquitin transferase SP1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SP1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

E3 ubiquitin-protein ligase involved in the regulation of protein import in the chloroplast. Associates with TOC complexes and mediates ubiquitination of TOC components, promoting their degradation via the ubiquitin-proteasome system (UPS). Plays a role in the reorganization of the TOC machinery. Involved in a mechanism that regulates plastid biogenesis via UPS. Promotes stress tolerance by depleting the chloroplast protein import apparatus, which limits photosystem assembly and the potential for reactive oxygen species (ROS) formation. May act as negative regulator of programmed cell death (PCD) during biotic stress.

Gene References into Functions

In this study, we identified the Arabidopsis RING-type E3 ubiquitin ligase SP1 as a peroxisome membrane protein that regulates peroxisome biogenesis. PMID:27799549
SP1 protein (At1g63900) associated with TOC (translocon at the outer envelope of chloroplasts) complexes and mediated ubiquitination of TOC components, promoting degradation; mutant sp1 plants performed developmental transitions involving plastid proteome changes inefficiently, indicating requirement for reorganization of TOC machinery PMID:23118188

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Subcellular Location

Plastid, chloroplast outer membrane; Multi-pass membrane protein.

Database Links

UNIGENE: At.22411

KEGG: ath:AT1G63900

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