Made-to-order (12-14 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Value-added Deliverables
① 200ug * antigen (positive control); ② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection; ② ELISA titer can be guaranteed 1: 64,000; ③ WB validation with antigen can be guaranteed positive;
E3 SUMO protein ligase involved in regulation processes. Mediates SUMO/ attachment to PHR1, a MYB transcriptional activator controlling the phosphate deficiency responses. Functions as an upstream negative regulator of salicylic acid (SA) accumulation and subsequent SA-mediated systemic acquired resistance (SAR) signaling. Probably not involved in jasmonic acid (JA)-mediated defense response. Participates in abiotic stress-induced sumoylation. Controls heat shock-induced SUMO1 and SUMO2 conjugation and facilitates basal thermotolerance. Involved in freezing tolerance by mediating sumoylation of ICE1, a transcription activator of the cold signaling regulator CBF3/DREB1A. Acts as positive regulator of drought stress tolerance. Acts as floral repressor that promotes FLC expression by repressing FLD activity through sumoylation. Acts as negative regulator of abscisic acid (ABA) signaling through ABI5 sumoylation. Mediates sumoylation of SCE1, GTE3 and GTE5. Functions as negative regulator of SnRK1 signaling through sumoylation of several components of the SnRK1 complex.
Gene References into Functions
Arabidopsis thaliana SIZ1 controls nuclear localization of Nitrate Reductases proteins, and ammonium negatively regulates their levels.PMID:29662028
SIZ1 suppresses an SNC1-dependent resistance response at both normal and high temperatures. At the same time, SIZ1 amplifies the dark and high temperature growth response, likely via COP1 and upstream of gene regulation by PIF4 and BRZ1.PMID:29357355
The SnRK1 complex is SUMOylated on multiple subunits and identify SIZ1 as the E3 Small Ubiquitin-like Modifier (SUMO) ligase responsible for this modification.PMID:26662259
regulates seed dormancy and germinationPMID:27130140
SUMO-conjugated proteins accumulate in response to high doses of sugar in a SIZ1-dependent manner.PMID:26468507
The methylation level of the Arabidopsis genome, including transposons, was significantly lower in the siz1-2 mutant than in wild-type plants. CMT3 was sumoylated by the E3 ligase activity of AtSIZ1 through a direct interaction.PMID:26398805
negatively affects stomatal closure and drought tolerance through accumulation of salicylic acidPMID:22963672
This study establishes that the diverse properties characteristic of SIZ1 are associated with specific domains and that they can be separated.PMID:20404572
The AtSIZ1 mutation is correlated with an overall decrease in alternative respiration and with a low carbohydrate content to maintain the carbon to nitrogen ratio in siz1-2 mutants.PMID:22732219
SIZ1 sustains the stability and normal function of the mature female gametophyte which is necessary for pollen tube guidance.PMID:22253727
The expression and enzymatic activity of Cu/ZnSOD1 (CSD1) in shoots of the siz1 mutant under excess Cu, was examined.PMID:21897129
These data suggest that SIZ1-mediated sumoylation is involved specifically in copper homeostasis and tolerance in planta.PMID:21632972
AtSIZ1 positively controls nitrogen assimilation by promoting sumoylation of nitrate reductases in Arabidopsis.PMID:21772271
SIZ1 facilitates the auxin-regulated root architecture remodeling that occurs in response to Pi deficiency.PMID:21156857
The salicylic acid-accumulating mutant siz1 exhibits sensitivity to chilling and freezing conditions.PMID:19959255
These results indicate that SIZ1 regulates cell growth and plant development with regulation of salicylic acid accumulation.PMID:20007967
The various domains of SIZ1 make unique contributions to the plant's ability to cope with its environment.PMID:19837819
SIZ1 and sumoylation facilitate basal thermotolerance through processes that are salicylic acid independent.PMID:17041025
SIZ1 is required for salicylic acid and PAD4-mediated R gene signalling, which in turn confers innate immunity in Arabidopsis.[SIZ1]PMID:17163880
SIZ1 is a SUMO E3 ligase that facilitates conjugation of SUMO to protein substrates.PMID:17416732
SIZ1 regulates Arabidopsis growth and that this SUMO E3 ligase plays a role in drought stress response likely through the regulation of gene expression.PMID:17905899
Results suggest that SIZ1 is a floral repressor that not only represses the SA-dependent pathway, but also promotes FLC expression by repressing FLD activity through sumoylation, which is required for full FLC expression in a FRIGIDA background.PMID:18069938