RAD50 Antibody

Code: CSB-PA019262XA01SVG

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

P12753

Target Names

RAD50

Alternative Names

RAD50 antibody; YNL250W antibody; N0872 antibody; DNA repair protein RAD50 antibody; EC 3.6.-.- antibody; 153 kDa protein antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAD50 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

Involved in DNA double-strand break repair (DSBR). The rad50/mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific exonuclease activity. Rad50 provides ATP-dependent control of mre11 by unwinding and/or repositioning DNA ends into the mre11 active site.

Gene References into Functions

Data demonstrate that the dimerization state of the Rad50 hook domain exerts diverse influences on Mre11 complex function. PMID:25601756
cohesin loading at replication sites depends on the structural features of Rad50 that are important for bridging sister chromatids, including the CXXC hook domain and the length of the coiled-coil extensions PMID:22885006
deletion of RAD50 may block the major pathway of nonhomologous integration into a non-repetitive chromosomal locus; Rad50 may be involved in facilitating nonhomologous integration of both ends of the integrating DNA into a single chromosomal locus PMID:21512733
amino acid substitutions in the rad50 ATP binding domain have effects on DNA double strand break repair PMID:15546877
The Mre11/Rad50/Xrs2 (MRX) complex is essential for joining of incompatible ends by non-homologous end-joining during DNA repair, and the adenosine triphosphate (ATP)-dependent activities of Rad50 are critical for this process. PMID:16043424
Rad50 is required for the induction of recombination between homologous chromosomes in cells in the vegetative growth state upon exposure to methyl methanesulfonate. PMID:17507781
The Rad50/Mre11/Xrs2 complex is responsible for rapid processing of most damaged ends into substrates that subsequently undergo recombinational repair. PMID:19763170