PDPK1 Antibody

Code: CSB-PA894967XA01DOA

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Arabidopsis thaliana

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

Q9XF67

Target Names

PDPK1

Alternative Names

PDPK1 antibody; PDK1 antibody; At5g04510 antibody; T32M21.110 antibody; 3-phosphoinositide-dependent protein kinase 1 antibody; AtPDK1 antibody; EC 2.7.11.1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PDPK1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

May couple lipid signals to the activation-loop phosphorylation of several protein kinases of the so-called AGC kinase family. Interacts via its pleckstrin homology domain with phosphatidic acid, PtdIns3P and PtdIns(3,4)P2 and to a lesser extent with PtdIns(4,5)P2 and PtdIns4P. May play a general role in signaling processes controlling the pathogen/stress response, polar auxin transport and development. Transphosphorylates the AGC protein kinases OXI1/AGC2-1, PK1/S6K1, PK19/S6K2 and PID resulting in their activation.

Gene References into Functions

PDK1 undergoes autophosphorylation at several sites; mutation of Ser-276 to Ala resulted in enzyme with no detectable autophosphorylation; other sites important for autophosphorylation &/or activity were Asp-167, Thr-176, & Thr-211 PMID:16125835

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Subcellular Location

Cytoplasm. Membrane; Peripheral membrane protein.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, PDPK1 subfamily

Tissue Specificity

Ubiquitous.

Database Links

UNIGENE: At.4924

KEGG: ath:AT5G04510

STRING: 3702.AT5G04510.1

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