The icarapin polyclonal antibody is generated using a recombinant Apis mellifera carnica icarapin protein segment (encompassing amino acids 20-223) as the immunogen. This segment is inoculated to a rabbit for immunization, leading to an antibody response. Serum collected from the rabbit contains polyclonal antibodies, which are subsequently purified via affinity chromatography. The icarapin antibody's functional efficacy is further confirmed through ELISA and WB assays, attesting to its suitability for detecting Apis mellifera carnica icarapin protein. The icarapin (Api m 10) is a novel IgE-binding bee venom protein responsible for the major allergenic response to bee stings.
UNIGENE: Ame.1095
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