IPL1 Antibody

Code: CSB-PA327918XA01SVG

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0.1ml

0.1ml1ml

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Species Reactivity: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

P38991

Target Names

IPL1

Alternative Names

IPL1 antibody; YPL209C antibody; Spindle assembly checkpoint kinase antibody; EC 2.7.11.1 antibody; Aurora kinase antibody; Increase-in-ploidy protein 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) IPL1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

Required for high-fidelity chromosome segregation during the later part of each cell cycle. Acts in opposition to the phosphatase PP1. Has a role in attaching the kinetochores to the microtubules and ensuring that sister kinetochores connect to opposite poles. The promotion of bi-orientation is achieved by selectively detaching kinetochore-microtubule attachments that are not under tension. Phosphorylates histone H3 to form H3S10ph during mitosis and meiosis. Phosphorylates RGD1 in vitro.

Gene References into Functions

Decatenation and condensation defects lead to spindle stabilization during cytokinesis, allowing bridge detection by Aurora B. PMID:27111841
Shedding is regulated by the conserved protein kinase Ipl1/Aurora-B and promotes the subsequent assembly of a kinetochore. PMID:26157162
increase in Aurora B activity causes premature collapse of the mitotic spindle by promoting instability of the spindle midzone. PMID:25201961
a novel and unexpected role for Aurora kinase in preventing the formation of spindles by Clb5-CDK (S-CDK) during meiotic prophase I PMID:24386320
protein phosphatase-1 and aurora protein kinase suppressors PMID:23894419
results suggest that activation of Aurora B kinase (Ipl1) by clustering either on chromatin or on microtubules is sufficient for chromosome biorientation PMID:23604256
The Ipl1/Aurora-dependent phosphorylation of Sli15 controls spindle function by excluding the CPC from spindle regions engaged in microtubule polymerization. PMID:21727193
Although the Dam1 kinetochore complex has been implicated as a key downstream target, we found the existence of unidentified Ipl1 sites on Dam1 or additional important substrates that regulate both microtuble detachment and the checkpoint. PMID:16327780
The overexpression of Glc7 regulatory subunits can titrate Glc7 away from relevant Ipl1 targets and thereby suppress ipl1-321 cells by restoring the balance of phosphatase/kinase activity. PMID:16537909
Study finds that the Aurora B kinase Ipl1 regulates kinetochore-microtubule attachment during both meiotic divisions and that a complex known as the monopolin complex ensures that the protein kinase coorients sister chromatids during meiosis I. PMID:17289568
We suggest that centromeric Ipl1 ensures the continued centromeric presence of active Rts1/PP2A, which in turn locally protects cohesin and cohesion. PMID:17371833
Tousled has a bona fide kinase-independent role in the activation of Aurora B kinases in vivo PMID:18334486
Nbl1p colocalizes and copurifies with the chromosomal passenger complex PMID:19158380
The budding Bim1p is regulated by Ipl1p-mediated multisite phosphorylation. Bim1p forms a stable complex with Ipl1p and is phosphorylated on a cluster of six Ser residues in the flexible linker connecting the calponin homology (CH) and EB1 domains. PMID:19667128
Ipl1 coordinates changes to meiotic chromosome structure with resolution of crossovers and cell cycle progression at the end of meiotic prophase PMID:19759266

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Associates with the mitotic spindle and on elongated and disassembling spindles. Also associated with the kinetochore.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily

Database Links

KEGG: sce:YPL209C

STRING: 4932.YPL209C

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