EBNA3 Antibody

Code: CSB-PA319032ZA01EFA

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Size:

20μl50μl100μl

US$83

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Species Reactivity: Epstein-Barr virus

Application: ELISA, WB

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Product Details
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Target Background

Product Details

Uniprot NO.

P12977

Target Names

EBNA3

Alternative Names

Epstein-Barr nuclear antigen 3 (EBNA-3) (EBV nuclear antigen 3) (Epstein-Barr nuclear antigen 3A) (EBNA-3A) (EBV nuclear antigen 3A), EBNA3

Species Reactivity

Epstein-Barr virus

Immunogen

Recombinant Epstein-Barr virus EBNA3 protein (1-138aa)

Immunogen Species

Epstein-Barr virus

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in Recombinant protein
All lanes: EBNA3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22.2 kDa
Observed band size: 33 kDa

Description

The development of the EBNA3 polyclonal antibody follows a well-defined procedure. It initiates with the repeated immunization of a rabbit using recombinant Epstein-Barr virus EBNA3 protein (1-138aa), continuing until an optimal antibody titer is achieved. Subsequently, the rabbit's blood is collected, and the antibodies are purified from the serum using protein G. The functionality of the resulting EBNA3 antibody is comprehensively assessed in ELISA and WB applications, affirming its specific binding with the Epstein-Barr virus EBNA3 protein.

The Epstein-Barr virus (EBV) EBNA3 proteins (EBNA3A, EBNA3B, and EBNA3C) play a crucial role in the regulation of viral gene expression and the transformation of B lymphocytes. They are involved in the modulation of host gene expression, cell cycle control, and immune evasion. EBNA3A and EBNA3C, in particular, contribute to maintaining the immortalization of infected B cells by inhibiting the function of certain tumor suppressor proteins.

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Target Background

Function(From Uniprot)

Plays an essential role for activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA3A probably contributes to a negative autoregulatory control loop.

Gene References into Functions

the polycomb repressor complex is essential for EBNA3A-mediated repression of STK39 Findings in this study provide new insights into the regulation of cellular genes by the transcription factor EBNA3A. PMID:29367247
These results indicate that EBNA3 proteins interact with multiple RBP/CSL domains, but only N-terminal domain interactions are required for lymphoblastoid cell line growth. PMID:21440926
EBNA-3 plays a role in facilitating the ligand-dependent AhR activation process PMID:16257957

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Subcellular Location

Host nucleus matrix. Note=Associated with the nuclear matrix.

Protein Families

Herpesviridae EBNA-3 family

Database Links

KEGG: vg:3783762

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