LIG1 Antibody

Code: CSB-PA671465XA01DOA

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Arabidopsis thaliana

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

Q42572

Target Names

LIG1

Alternative Names

LIG1 antibody; At1g08130 antibody; T23G18.1 antibody; T6D22.23DNA ligase 1 antibody; AtLIG1 antibody; EC 6.5.1.1 antibody; DNA ligase I antibody; Polydeoxyribonucleotide synthase [ATP] 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana LIG1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

Essential protein. DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Involved in repair of both single strand breaks (SSBs) and double strand breaks (DSBs). Required in the endosperm for embryogenesis, probably to repair DNA-breaks generated by DME.

Gene References into Functions

The AtLIG1 is an important component of the active DNA demethylation machinery. PMID:25906993
LIG1 is required for both single-nucleotide or long-patch base excision repair. PMID:21781197
In the study, we show that loss-of-function of the major DNA LIGASE I (AtLIG1) in Arabidopsis thaliana causes maternal effects in the endosperm, which is the seed tissue that nurtures embryo development. PMID:20023162
Nuclear or mitochondrial targeting of LIG1 is accomplished through an evolutionarily conserved translation initiation mechanism. [AtLIG1] PMID:16790030
DNA ligase 1 functions in both DNA replication and in repair of both ss and dsDNA strand breaks. PMID:19558640