crp Antibody

Code: CSB-PA833047XA01FSG

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

P9WMH3

Target Names

crp

Alternative Names

crp antibody; Rv3676CRP-like cAMP-activated global transcriptional regulator antibody; cAMP receptor protein antibody; CRP antibody; cAMP regulatory protein antibody

Raised in

Rabbit

Species Reactivity

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Immunogen

Recombinant Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) crp protein

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

Global transcriptional regulator that complexes with cAMP and binds to specific DNA promoter sites, causing DNA-bending, to regulate transcription. cAMP improves binding to specific DNA sequences, probably by altering protein conformation. The CRP regulon is predicted to contain about 115 genes. Some genes are activated by CRP (rpfA, whiB1) while others are repressed (fadD10). There are 2 CRP-binding sites in the promoter of whiB1, at low concentrations of CRP with or without cAMP transcription of whiB1 is enhanced via site CRP1, then repressed as site CRP2 is filled.

Gene References into Functions

TopoI is required for the Mycobacterium tuberculosis growth. PMID:24593153
Mycobacterium tuberculosis cAMP receptor protein can function as a classical transcription factor in M. tuberculosis, though this occurs at only a subset of cAMP receptor protein binding sites. PMID:24957601
Data show that M. tuberculosis cAMP receptor protein (CRP) has evolved several distinct characteristics, compared with the Escherichia coli CRP paradigm, to allow it to regulate gene expression against a background of high concentrations of cAMP. PMID:20028978
Rv3676 is a direct regulator of rpfA expression, and because rpfA codes for a resuscitation promoting factor this may implicate Rv3676 in reactivation of dormant M. tuberculosis infections. PMID:15882420
These results provide the first direct evidence for cAMP binding to a transcription factor in M. tuberculosis, suggesting a role for cAMP signal transduction in M. tuberculosis and implicating CRP(Mt) as a cAMP-responsive global regulator. PMID:16267303
The CRP/FNR family transcription factor from M. tuberculosis H37Rv has been crystallized; the structure of the protein might provide insight into transcriptional regulation in the pathogen by this protein. PMID:16946467
At 6-10 microM concentrations of cAMP Rv3676 shows positive cooperativity; at 10 microM cAMP the protein exists in the most open conformation. Rv3676 could bind specifically to the putative CRP/FNR nucleotide sequence elements. PMID:17702648
Comparison of the structures of apo MtbCRP and DNA-bound EcCRP shows how DNA binding would be inhibited in the absence of cAMP and supports a mechanism involving functional asymmetry in apoCRP. PMID:19193643
Structural insights into the mechanism of the allosteric transitions of Mycobacterium tuberculosis cAMP receptor protein. PMID:19740754