Chc Antibody

Code: CSB-PA333472XA01DLU

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Size:

20μl50μl100μl

US$83

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Species Reactivity: Drosophila melanogaster (Fruit fly)

Application: ELISA, WB

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Product Details
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Target Background

Product Details

Uniprot NO.

P29742

Target Names

Chc

Alternative Names

Clathrin heavy chain Chc CG9012

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Chc protein (1-260aa)

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: recombinant protein
All lanes: Chc Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa

Description

The Chc polyclonal antibody is developed by immunizing a rabbit with a recombinant Drosophila melanogaster (Fruit fly) Chc protein Chc protein segment (amino acids 1-260). The resulting serum contains polyclonal antibodies, which are purified using affinity chromatography. The functionality of the Chc antibody is determined via ELISA and WB assays, affirming its effectiveness in detecting the Drosophila melanogaster (Fruit fly) Chc protein Chc protein in experimental conditions.

In Drosophila melanogaster (fruit fly), the Chc (Clathrin heavy chain) protein is a critical component of the clathrin protein complex, primarily involved in endocytosis, receptor-mediated internalization, and intracellular trafficking. Chc participates in the formation of clathrin-coated vesicles, which are instrumental in the uptake of substances from the extracellular environment through endocytosis. Notably, in neurons, Chc contributes to the recycling of synaptic vesicles, playing a key role in maintaining synaptic function.

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Target Background

Function(From Uniprot)

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

Gene References into Functions

Bic-D is also required to transport Chc mRNA into the oocyte and for transport and proper localization of Chc protein to the oocyte cortex. PMID:24718986
Results report a Drosophila disabled (DAB)mutant revealing a novel role for DAB proteins in chemical synaptic transmission, and support a role for clathrin-mediated endocytosis in rapid clearance of neurotransmitter release sites. PMID:21606364
Bicaudal-D binds clathrin heavy chain to promote its transport and augments synaptic vesicle recycling. PMID:20111007
chc mRNA and protein are highly expressed from early stages of embryogenesis onwards, consistent with genetic studies predicting a maternal contribution of the gene function PMID:19577664
Chc is critical for synaptic vesicle recycling and in the absence of the protein, bulk retrieval mediates massive synaptic membrane internalization. PMID:18762582

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Subcellular Location

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic face of coated pits and vesicles.

Protein Families

Clathrin heavy chain family

Database Links

UNIGENE: Dm.3174

KEGG: dme:Dmel_CG9012

STRING: 7227.FBpp0073966

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